干旱区生态环境知识资源中心(Arid): Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2019.07028
	Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry
	Zhang Hao; Wu Yinliang; Zhang Yiwen; Xu Xiuqin; Xu Feng
通讯作者	Zhang, H
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2019
卷号	37 期号:12 页码:1314-1320
英文摘要	An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantitative determination of ten cephalosporins in bee products, including honey, royal jelly, and lyophilized royal jelly powder, has been established. The samples were extracted with an acetonitrile-water (80:20, v/v) solution. After purification by solid phase extraction using an Oasis PRIME HLB cartridge, the extract was blown to dryness under a stream of nitrogen gas and then re-dissolved in 1 mL 0.1% (v/v) formic acid solution and methanol (95:5, v/v). The samples were separated on an Acquity UPLC BEH C18 column, using a mixture of 0.1% (v/v) formic acid solution and methanol as the mobile phase under gradient elution. The analysis was carried out using a positive electrospray ion source in the multiple reaction monitoring mode. The matrix-matched external standard method was applied to quantitative analysis. Good linear relationships were obtained for the ten cephalosporins in certain concentration ranges, and the correlation coefficients were more than 0.999. The limits of detection and limits of quantification for the ten cephalosporins were in the ranges 0.15-1.5 mu g/kg and 0.5-5 mu g/kg, respectively. The recoveries for the analytes in the bee products were in the range of 75.0% to 89.8%, with relative standard deviations of 1.4% to 4.6%. This method is characterized by a short analysis time and is suitable for the determination of cephalosporins in different bee products by virtue of its simplicity and reliability.
英文关键词	solid phase extraction (SPE) ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) cephalosporins bee products
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000502592500009
WOS关键词	DISPERSIVE EXTRACTION ; MILK ; CEFOTAXIME ; RESIDUES ; ANTIBIOTICS
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333984
作者单位	[Zhang Hao; Wu Yinliang; Xu Xiuqin; Xu Feng] Ningbo Acad Agr Sci, Lab Qual & Safety Risk Assessment Agr Prod Ningbo, Minist Agr & Rural Affairs, Ningbo 315040, Zhejiang, Peoples R China; [Zhang Yiwen] Beijing Inst Metrol, Beijing 100012, Peoples R China
推荐引用方式 GB/T 7714	Zhang Hao,Wu Yinliang,Zhang Yiwen,et al. Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry[J],2019,37(12):1314-1320.
APA	Zhang Hao,Wu Yinliang,Zhang Yiwen,Xu Xiuqin,&Xu Feng.(2019).Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,37(12),1314-1320.
MLA	Zhang Hao,et al."Determination of ten cephalosporins in bee products by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 37.12(2019):1314-1320.