干旱区生态环境知识资源中心(Arid): Determination of 11 sulfonamides in pork by two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2019.04005
	Determination of 11 sulfonamides in pork by two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry
	Liu Peiyong; Zhang Hui; Mi Zhijin; Zhang Liangcheng; Zhang Guangren
通讯作者	Liu, PY
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2019
卷号	37 期号:10 页码:1098-1104
英文摘要	A method based on two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of 11 common sulfonamides in pork. The samples were extracted first with ethyl acetate containing 2% (v/v) formic acid and then with acetone for the second step. The supernatant was degreased with n-hexane and cleaned using an Oasis MCX SPE column. Finally, nitrogen was blown over the obtained mixture, and the mixture was filtered for conducting HPLC-MS/MS analysis. The sulfonamides were detected in the multiple reaction monitoring positive ion mode, and quantification was performed by employing the external standard method with matrix calibration curves. All the 11 sulfonamides demonstrated good linearity in the range of 20-400 mu g/L, with the correlation coefficient (r(2)) higher than 0.99. The limits of detection (LODs; S/N = 3) and limits of quantification (LOQs; S/N = 10) were found to be in the ranges of 0.1 - 1.0 mu g/kg and 0.2 - 3.0 mu g/kg, respectively. Furthermore, the results indicated that the average recoveries of the 11 sulfonamides ranged from 79.3% to 105.5% at three spiked levels of 50, 100, and 200 mu g/kg, with the relative standard deviations (RSDs) in the range of 1.3% - 11.6% (n = 6). This method showed higher extraction efficiency than the one-step liquid-liquid extraction, and the detection sensitivity was highly improved.
英文关键词	high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) two-step liquid-liquid extraction solid phase extraction (SPE) sulfonamides veterinary drug residues pork
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000502590800011
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333983
作者单位	[Liu Peiyong; Zhang Hui; Mi Zhijin; Zhang Liangcheng; Zhang Guangren] Dazhou Food & Drug Inspect Inst, Dazhou 635000, Peoples R China
推荐引用方式 GB/T 7714	Liu Peiyong,Zhang Hui,Mi Zhijin,et al. Determination of 11 sulfonamides in pork by two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry[J],2019,37(10):1098-1104.
APA	Liu Peiyong,Zhang Hui,Mi Zhijin,Zhang Liangcheng,&Zhang Guangren.(2019).Determination of 11 sulfonamides in pork by two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,37(10),1098-1104.
MLA	Liu Peiyong,et al."Determination of 11 sulfonamides in pork by two-step liquid-liquid extraction-solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 37.10(2019):1098-1104.