干旱区生态环境知识资源中心(Arid): Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2019.03014
	Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid chromatography-tandem mass spectrometry
	Wang Junhu; Qiao Yongsheng; Wang Jian; Zhu Yuan
通讯作者	Zhu, Y
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2019
卷号	37 期号:9 页码:963-968
英文摘要	A method for the rapid determination of biotoxin (bongkrekic acid) in the Liushenqu was established using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with methanol by ultrasonication, then the pH was adjusted to 8 using ammonia. After filtration, the extract was purified using an Oasis Max strong anion exchange resin column. The Waters HSS T3 column (100 mmx2.1 mm, 1.8 mu m) was used for the UPLC-MS/MS analysis. The mobile phase was acetonitrile-10 mmol/L ammonium formate solution (containing 0.1% (v/v) formic acid). MS analysis was performed using an electrospray ionization (ESI) source in the negative and multiple reaction monitoring (MRM) modes. Under the optimum conditions, the linear range of bongkrekic acid was 0.5-100 mu g/L (correlations coefficient (R-2)>0.99). The recoveries of the bongkrekic acid were 80.6%-85.3%. The intra-day and inter-day relative standard deviations (RSDs) were in the range of 4.2%-6.8% and 8.2%-13.2%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.4 mu g/kg and 1.2 mu g/kg, respectively. The results showed that bongkrekic acid residues were detected in Liushenqu, thereby confirming the supporting role of our method for the risk monitoring of biotoxins in health foods and Chinese herbal medicines. This method is simple, easy, sensitive, and suitable for the determination of the bongkrekic acid residues in Liushenqu.
英文关键词	ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) biotoxin bongkrekic acid Liushenqu
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000502590000006
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333982
作者单位	[Wang Junhu; Qiao Yongsheng; Wang Jian; Zhu Yuan] Taizhou Inst Food & Drug Control, Taizhou 225300, Peoples R China
推荐引用方式 GB/T 7714	Wang Junhu,Qiao Yongsheng,Wang Jian,et al. Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid chromatography-tandem mass spectrometry[J],2019,37(9):963-968.
APA	Wang Junhu,Qiao Yongsheng,Wang Jian,&Zhu Yuan.(2019).Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid chromatography-tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,37(9),963-968.
MLA	Wang Junhu,et al."Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid chromatography-tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 37.9(2019):963-968.