干旱区生态环境知识资源中心(Arid): Simultaneous determination of 120 veterinary antibiotics in meat by combined pretreatment system coupled with ultra-performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2018.10003
	Simultaneous determination of 120 veterinary antibiotics in meat by combined pretreatment system coupled with ultra-performance liquid chromatography-tandem mass spectrometry
	Bian Hua; Qin Yu; Yu Chenghua; Zhang Kai; Wang Chengping; Lin Yikan; Yang Baogang; Ge Yu
通讯作者	Ge, Y
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2019
卷号	37 期号:2 页码:162-176
英文摘要	A comprehensive pretreatment system was developed to simultaneously extract and purify 120 veterinary antibiotics, possessing various physicochemical properties in eight categories. This system was coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the detection of the antibiotics. The samples were dissolved and dispersed by a Na(2)EDTA-Mcllvaine buffer, extracted with acetonitrile containing 1. 5% (v/v) formic acid, and cleaned using an Oasis PRiME HLB SPE system followed by n-hexane liquid purification. The separation of residue targets was performed on an Atlantis T3 column (100 mmx2. 1 mm, 3 m) with a gradient elution of 0. 1% (v/v) formic acid aqueous solution and acetonitrile as mobile phases and detected by UPLC-MS/MS with the multiple reaction monitoring (MRM) mode via Esr ionization. The pH of the extraction solvent and the purification method were optimized to promote the target recoveries. All targets showed good linear ranges from 1. 0 to 50. 0 mu g/L, while all their correlation coefficients (r(2)) were higher than 0. 995 3, including r(2) values of 89 targets being over 0. 999 0. The limits of quantification (LOQs) of seven targets were 10. 0 mu g/kg; 21 targets, 5. 0 g/kg; and the remaining 92 targets, not higher than 2. 0 g/kg. The average recoveries on three spiked levels (low, medium, and high) for all targets ranged from 71. 5% -109. 2%, with RSD ranging from 0. 6% -15. 3%. The combined system, exhibiting satisfying recovery and stable repeatability, can be suitably employed for the simultaneous determination of multiple veterinary antibiotics in animal -derived meat products.
英文关键词	combined pretreatment system ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) veterinary antibiotics meat
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000502570600006
WOS关键词	SOLID-PHASE EXTRACTION ; RESIDUES ; QUECHERS ; MUSCLE ; DRUGS ; MILK
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333980
作者单位	[Bian Hua; Qin Yu; Yu Chenghua; Wang Chengping; Lin Yikan; Yang Baogang; Ge Yu] Shanghai Inst Qual Inspect & Tech Res, Shanghai 200233, Peoples R China; [Zhang Kai] Univ Shanghai Sci & Technol, Coll Med Equipment & Food, Shanghai 200093, Peoples R China
推荐引用方式 GB/T 7714	Bian Hua,Qin Yu,Yu Chenghua,et al. Simultaneous determination of 120 veterinary antibiotics in meat by combined pretreatment system coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J],2019,37(2):162-176.
APA	Bian Hua.,Qin Yu.,Yu Chenghua.,Zhang Kai.,Wang Chengping.,...&Ge Yu.(2019).Simultaneous determination of 120 veterinary antibiotics in meat by combined pretreatment system coupled with ultra-performance liquid chromatography-tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,37(2),162-176.
MLA	Bian Hua,et al."Simultaneous determination of 120 veterinary antibiotics in meat by combined pretreatment system coupled with ultra-performance liquid chromatography-tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 37.2(2019):162-176.