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	In vitro antioxidant capacity of Euphorbia retusa Forssk. from Algerian desert
	Lahmadi, Selwa; Belhamra, Mohamed; Karoune, Samira; Kechebar, Mohamed Seif Allah; Bensouici, Chawki; Kashi, Imad; Mizab, Ouahiba; Ksouri, Riadh
通讯作者	Lahmadi, S
来源期刊	JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
ISSN	0719-4250
出版年	2019
卷号	7 期号:5 页码:356-366
英文摘要	Context: Euphorbia retusa Forssk. is a medicinal plant, which traditionally is used in Algerian Sahara for treatment of infantile eczema, warts and trichiasis. Aims: To evaluate phenolic composition and antioxidant capacity of methanolic extracts from stems, leaves and seeds of Euphorbia retusa. Methods: Total phenolic and flavonoids were analyzed by Folin-Ciocalteu and aluminum chloride assays, respectively using gallic acid (GA) and quercetin (Q) as standard compounds. Antioxidant capacity was studied in vitro by using DPPH and ABTS cation radical scavenging, cupric-reducing antioxidant capacity (CUPRAC) and reducing power assays, followed by identification of phenolic compounds of seeds by mean of high-performance liquid chromatography (HPLC-DAD). Results: Methanolic extracts of E. retusa seeds had the highest total phenolic and flavonoid contents with 315.50 +/- 1.41 mu g GA equivalent/ mg dry extract and 91.83 +/- 8.18 mu g Q equivalent/mg dry extract, respectively. Identification of phenolic compounds from seeds revealed that epigallocatechin 3-O- gallate was the major flavonoid. This organ showed the highest activity in the assays of DPPH (IC50 = 7.20 +/- 0.25 mu g/ mL), ABTS (IC50 < 6.25 mu g/ mL), CUPRAC (A(0.5) = 10.64 +/- 0.99 mu g/mL) and reducing power (A(0.5)= 11.84 +/- 1.72 mu g/ mL). Conclusions: Euphorbia retusa seeds have an antioxidant capacity that could be useful in pharmacological and medicinal fields.
英文关键词	antioxidant Euphorbia retusa HPLC-DAD phenolics
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000483848600005
WOS关键词	PHENOLIC-COMPOUNDS ; L. ; ORGANS ; ACIDS
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
Scopus学科分类	Ctr Rech Sci & Tech Reg Arides, Campus Univ,BP 1682 RP, Biskra 07000, Algeria.
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333783
作者单位	[Lahmadi, Selwa; Belhamra, Mohamed] Univ Mohamed Khider, Dept Agron, Lab Diversite Ecosyst & Dynam Syst Prod Agr Zones, Biskra 07000, Algeria; [Lahmadi, Selwa; Karoune, Samira; Kechebar, Mohamed Seif Allah; Mizab, Ouahiba] Ctr Rech Sci & Tech Reg Arides, Campus Univ,BP 1682 RP, Biskra 07000, Algeria; [Bensouici, Chawki; Kashi, Imad] Ctr Rech Biotechnol, Ali Mendjli Nouvelle Ville UV 03, Constantine 25000, Algeria; [Ksouri, Riadh] Ctr Biotechnol Borj Cedria, Lab Plantes Aromat & Med, BP 901, Hammam Lif 2050, Tunisia
推荐引用方式 GB/T 7714	Lahmadi, Selwa,Belhamra, Mohamed,Karoune, Samira,et al. In vitro antioxidant capacity of Euphorbia retusa Forssk. from Algerian desert[J],2019,7(5):356-366.
APA	Lahmadi, Selwa.,Belhamra, Mohamed.,Karoune, Samira.,Kechebar, Mohamed Seif Allah.,Bensouici, Chawki.,...&Ksouri, Riadh.(2019).In vitro antioxidant capacity of Euphorbia retusa Forssk. from Algerian desert.JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH,7(5),356-366.
MLA	Lahmadi, Selwa,et al."In vitro antioxidant capacity of Euphorbia retusa Forssk. from Algerian desert".JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH 7.5(2019):356-366.