干旱区生态环境知识资源中心(Arid): Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran

Arid

DOI	10.15171/PS.2019.11
	Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran
	Elyasifar, Babak; Jafari, Sevda; Hallaj-Nezhadi, Somayeh; Chapeland-Ieclerc, Florence; Ruprich-Robert, Gwenael; Dilmaghani, Azita
通讯作者	Dilmaghani, A
来源期刊	PHARMACEUTICAL SCIENCES
ISSN	1735-403X
EISSN	2383-2886
出版年	2019
卷号	25 期号:1 页码:70-77
英文摘要	Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered in drug innovation and control of plant pathogens. Salt deserts in Semnan province are of the most permanent hypersaline areas in the North of Iran. Despite the importance of these areas, there is no scientific report regarding the biodiversity and potency of their halophilic bacteria. Thus, aforementioned areas were selected to detect the halophilic bacteria. Methods: Here, seven strains were isolated and cultured on their molecular and biochemical properties were characterized. To determine the antibiotic potency of the isolates, agar well diffusion method was conducted. Phylogenetic analysis was done to reveal the isolates relationship with previously known strains. Results: As a result, growth of the strains in the medium containing 5 to 20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. Catalase activity of all strains was positive. The results represented that D6A, Dar and D8B have antimicrobial effects against different plant and human pathogens. Phylogenic tree analysis also showed that two strains of D6A and Dar are belonged to Bacillus subtilis and D8B is belonged to Virgibacillus olivae. The bacteria extracts were evaluated for their antifungal and antibacterial activities on human and Plant pathogenic strains. The MIC of the extract B. subtilis against was found active against human pathogenic fungi and Plant pathogenic bacteria and fungi, ranging from 12.5 to 25 mu g/mL. Conclusion: This study highlights the therapeutic and prophylactic potential of B. subtilis extracts as antibacterial and antifungal agents.
英文关键词	Halophilic bacteria Antimicrobial Pathogen Dagh Biarjmand and Hai Aligholi salt deserts
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000481499300011
WOS关键词	DIVERSITY ; WATER ; NOV.
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
Scopus学科分类	Tabriz Univ Med Sci, Dept Pharmaceut Biotechnol, Fac Pharm, Tabriz, Iran.
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333744
作者单位	[Elyasifar, Babak; Jafari, Sevda; Hallaj-Nezhadi, Somayeh; Dilmaghani, Azita] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran; [Elyasifar, Babak; Jafari, Sevda; Hallaj-Nezhadi, Somayeh; Dilmaghani, Azita] Tabriz Univ Med Sci, Dept Pharmaceut Biotechnol, Fac Pharm, Tabriz, Iran; [Chapeland-Ieclerc, Florence; Ruprich-Robert, Gwenael] Univ Paris 05, Sorbonne Paris Cite, Inst Energies Demain, UMR 8236, F-75205 Paris, France
推荐引用方式 GB/T 7714	Elyasifar, Babak,Jafari, Sevda,Hallaj-Nezhadi, Somayeh,et al. Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran[J],2019,25(1):70-77.
APA	Elyasifar, Babak,Jafari, Sevda,Hallaj-Nezhadi, Somayeh,Chapeland-Ieclerc, Florence,Ruprich-Robert, Gwenael,&Dilmaghani, Azita.(2019).Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran.PHARMACEUTICAL SCIENCES,25(1),70-77.
MLA	Elyasifar, Babak,et al."Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran".PHARMACEUTICAL SCIENCES 25.1(2019):70-77.