干旱区生态环境知识资源中心(Arid): Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress

Arid

DOI	10.3934/genet.2019.2.17
	Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress
	Wang, Xiaojuan; Wu, Jianghong; Yang, Zhongren; Zhang, Fenglan; Sun, Hailian; Qiu, Xiao; Yi, Fengyan; Yang, Ding; Shi, Fengling
通讯作者	Shi, FL
来源期刊	AIMS GENETICS
ISSN	2377-1143
出版年	2019
卷号	6 期号:2 页码:17-35
英文摘要	Kochia prostrata is a good forage plant, which has important economic and ecological value in arid and semi-arid regions of China. Drought is one of the main factors affecting its productivity. At present, there are few studies on the mechanism of drought resistance. In order to reveal the changes of physiological and biochemical indexes, stomatal structure and gene expression profiles of Kochia prostrata under drought treatment, the classical determination method and high-throughput Illumina Hiseq sequencing platform were applied to the control group (CK) and drought treatment group of Kochia prostrata. The results showed that under the condition of moderate to mild drought stress, the SOD activity reached the maximum value of 350.68 U/g min on the 5th day of stress, and under the condition of severe drought stress, the SOD activity reached the maximum on the 2nd day of stress. The accumulation of Proline remained at a high level on the 5th day of stress, and there was at least one epidermal cell interval between the two adult stomatal of the leaf epidermis, so that the evaporation shell of each stomatal did not overlap, it ensures the efficient gas exchange of the stomatal, indicating that the Kochia prostrata has strong drought resistance. A total of 1,177.46 M reads were obtained by sequencing, with a total of 352.25 Gbp data and Q30 of 85%. In the differential gene annotation to the biological process (BP), a total of 261 GO terms were enriched in the up-regulated genes, and a total of 231 GO terms were enriched in the down-regulated genes. The differentially expressed genes (DEGs) were obtained in 27 KEGG metabolic pathways, which laid a foundation for revealing the molecular mechanism of drought tolerance.
英文关键词	Kochia prostrate drought stress stomatal transcription factors physiological changes
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000478571800003
WOS关键词	SALICYLIC-ACID ; GENE ; ARABIDOPSIS ; ADAPTATION ; EXPRESSION ; TOLERANCE ; PATHWAY ; KEGG
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333720
作者单位	[Yang, Zhongren; Zhang, Fenglan; Shi, Fengling] Inner Mongolia Agr Univ, Coll Grassland Resources & Environm Grass Resourc, Hohhot, Peoples R China; [Wang, Xiaojuan] ChiFeng Univ, Agr Sci Res Inst, Grass Resource Genet Breeding, Chifeng, Peoples R China; [Sun, Hailian; Qiu, Xiao; Yi, Fengyan; Yang, Ding] Chinese Acad Sci, Inner Mongolia Acad Agr & Anim Husb Sci, Inner Mongolia Grass Res Ctr, Grass Resource Genet Breeding, Beijing, Peoples R China; [Wu, Jianghong] Inner Mongolia Univ Nationalities, Coll Anim Sci & Technol, Tongliao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaojuan,Wu, Jianghong,Yang, Zhongren,et al. Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress[J],2019,6(2):17-35.
APA	Wang, Xiaojuan.,Wu, Jianghong.,Yang, Zhongren.,Zhang, Fenglan.,Sun, Hailian.,...&Shi, Fengling.(2019).Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress.AIMS GENETICS,6(2),17-35.
MLA	Wang, Xiaojuan,et al."Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress".AIMS GENETICS 6.2(2019):17-35.