干旱区生态环境知识资源中心(Arid): Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress: The Camelus dromedarius erythrocytes Se-GPx

Arid

DOI	10.1016/j.bcab.2019.01.038
	Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress: The Camelus dromedarius erythrocytes Se-GPx
	Chafik, Abdelbasset; Essamadi, Abdelkhalid; Celik, Safinur Yildirim; Solak, Kubra; Mavi, Ahmet
通讯作者	Chafik, A
来源期刊	BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
EISSN	1878-8181
出版年	2019
卷号	18
英文摘要	Camel lives most of its life under high environmental stress in the desert. Glutathione peroxidase plays a key role in protecting cells against oxidative stress. For the first time, selenium-dependent glutathione peroxidase (Se-GPx) was purified from camel erythrocytes, biochemically characterized, and some of its properties were studied. The enzyme was purified using ethanol-chloroform treatment, acetone precipitation and ion exchange chromatography. A purification fold of 33.72 with 0.19% yield were obtained. The native molecular weight of the enzyme was estimated to be about 69 kDa. On SDS-PAGE, the enzyme was composed of two different subunits with a molecular weight of approximately 53 and 21 kDa. An optimum temperature of 47 degrees C and an optimum pH of 7.2 were found. The activation energy was 41.71 kJ/mol. This enzyme was inhibited by thiol reagents, D,L-Dithiothreitol and beta-Mercaptoethanol, and was sensitive to bivalent cations. The enzyme had a general specificity toward hydroperoxides, and high specificity for reduced glutathione. The purified enzyme contained 3.06 mol of selenium per mol of protein. The K-m and V-max values for hydrogen peroxide and reduced glutathione were 0.72 and 1.58 mM, and 25.33 and 31.03 U/mg, respectively. The biochemical properties of camel Se-GPx were different comparing to those of mammalian species. Lower molecular weight, heterodimeric structure, higher optimum temperature, relatively lower optimum pH, lower content of selenium and higher affinity for hydrogen peroxide at low reduced glutathione concentration, these could be explained by the fact that camel is able to live in the intense environmental stress in the desert.
英文关键词	Camel Environmental stress Desert Glutathione peroxidase Biochemical properties
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000469474200008
WOS关键词	LIPID-PEROXIDATION ; HUMAN-PLASMA ; PURIFICATION ; LIVER ; ENZYME ; TRANSFERASE ; CADMIUM ; ELECTROPHORESIS ; INHIBITION ; COMPONENT
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333568
作者单位	[Chafik, Abdelbasset; Essamadi, Abdelkhalid] Univ Hassan First, Fac Sci & Technol, Team Appl Biochem & Toxicol, Lab Biochem & Neurosci, Settat 577, Morocco; [Celik, Safinur Yildirim] Bayburt Univ, Coll Educ, TR-69000 Bayburt, Turkey; [Solak, Kubra] Ataturk Univ, Inst Sci, Dept Nanosci & Nanoengn, TR-25240 Erzurum, Turkey; [Mavi, Ahmet] Ataturk Univ, Kazim Karabekir Educ Fac, Chem Educ, TR-25240 Erzurum, Turkey
推荐引用方式 GB/T 7714	Chafik, Abdelbasset,Essamadi, Abdelkhalid,Celik, Safinur Yildirim,et al. Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress: The Camelus dromedarius erythrocytes Se-GPx[J],2019,18.
APA	Chafik, Abdelbasset,Essamadi, Abdelkhalid,Celik, Safinur Yildirim,Solak, Kubra,&Mavi, Ahmet.(2019).Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress: The Camelus dromedarius erythrocytes Se-GPx.BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY,18.
MLA	Chafik, Abdelbasset,et al."Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress: The Camelus dromedarius erythrocytes Se-GPx".BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 18(2019).