干旱区生态环境知识资源中心(Arid): High-frequency multiple shoot induction from nodal segments and assessment of genetic homogeneity of micropropagated Atriplex halimus L.

Arid

	High-frequency multiple shoot induction from nodal segments and assessment of genetic homogeneity of micropropagated Atriplex halimus L.
	Vinod, Kumar; Sabah, AlMomin; Anisha, Shajan; Hamed, Al-Aqeel; Fadila, Al-Salameen; Sindhu, Nair
通讯作者	Vinod, K
来源期刊	RESEARCH JOURNAL OF BIOTECHNOLOGY
ISSN	2278-4535
出版年	2019
卷号	14 期号:5 页码:7-15
英文摘要	Atriplex halimus L. is a potentially useful plant in the arid region for animal feed, soil conservation and phytoremediation. Propagation of this plant through seeds is hindered by seed dormancy and a short viability period. Development of a robust tissue culture-based regeneration method would greatly facilitate rapid multiplication. In order to develop an efficient micropropagation method, we evaluated the effects of various growth regulators in the synthetic culture media. For initiation of the shoot multiplication process, surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of growth hormones such as 6-benzyladenine (BA) or thidiazuron (TDZ) alone or in combination with indole-3-acetic acid (IAA). The optimum response for shoot bud multiplication was observed on MS medium supplemented with both BA and TDZ. The shoot buds were then allowed to elongate and root. The plants were acclimatized, transplanted to soil and the regenerated plants were subjected to molecular analysis to determine their genetic homogeneity. Jaccard's coefficient and Nei and Li coefficient indicated high degree of genetic identity between the mother and the tissue-cultured plants. The results suggest suitability of this propagation technique for large-scale multiplication of A. halimus L.
英文关键词	Micropropagation Tissue culture Genetic variations ISSR Organogenesis
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000465955900002
WOS关键词	WATER-USE ; THIDIAZURON ; GROWTH ; COPPER
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333511
作者单位	[Vinod, Kumar; Sabah, AlMomin; Anisha, Shajan; Hamed, Al-Aqeel; Fadila, Al-Salameen; Sindhu, Nair] Kuwait Inst Sci Res, Biotechnol Program, Environm & Life Sci Res Ctr, Kuwait, Kuwait
推荐引用方式 GB/T 7714	Vinod, Kumar,Sabah, AlMomin,Anisha, Shajan,et al. High-frequency multiple shoot induction from nodal segments and assessment of genetic homogeneity of micropropagated Atriplex halimus L.[J],2019,14(5):7-15.
APA	Vinod, Kumar,Sabah, AlMomin,Anisha, Shajan,Hamed, Al-Aqeel,Fadila, Al-Salameen,&Sindhu, Nair.(2019).High-frequency multiple shoot induction from nodal segments and assessment of genetic homogeneity of micropropagated Atriplex halimus L..RESEARCH JOURNAL OF BIOTECHNOLOGY,14(5),7-15.
MLA	Vinod, Kumar,et al."High-frequency multiple shoot induction from nodal segments and assessment of genetic homogeneity of micropropagated Atriplex halimus L.".RESEARCH JOURNAL OF BIOTECHNOLOGY 14.5(2019):7-15.