干旱区生态环境知识资源中心(Arid): Genotoxic effect of Rhazya stricta leaf extract against larvae of Aedes aegypti

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	Genotoxic effect of Rhazya stricta leaf extract against larvae of Aedes aegypti
	Mohammed, Baeshen N.; Sanaa, Al-Attas G.; Mohamed, Ahmed M. M.; Yasir, Anwar; Abdul-Rahman, Faragalla A.; Hassan, Ramdan A.; Ayman, Elkady, I; Jazem, Mahyoub A.; Huda, Alabdali A.; Nabih, Baeshen A.
通讯作者	Yasir, A
来源期刊	RESEARCH JOURNAL OF BIOTECHNOLOGY
ISSN	2278-4535
出版年	2019
卷号	14 期号:4 页码:30-36
英文摘要	The biocontrol methods are extensively utilized for the control of pests. For this purpose, traditional plants are extensively used in Saudi Arabia. The predestined aim was the molecular evaluation of pesticidal effects of leave extract of a desert shrub Rhazya stricta (Decne) on larvae of Aedes aegypti (L.) (Diptera: Culicidae). Larvae of Aedes aegypti (L.) (Diptera: Culicidae) were exposed to different concentrations of Rhazya stricta aqueous leave extract (RSLE). Experimental approaches including comet assays, RAPD-PCR analysis and mitochondrial 16s rRNA gene analysis were carried out. The results showed promising lethal effects on third and fourth instar larvae by using stock solution. The percentages of adult insects emerging from their pupas ranged between 22-90% depending on the different concentrations used. The inhibitory concentration for adult mosquitoes' emergence reached about 0.057%. Phylogenic and RAPD analysis showed that these larvae are from 23 different genotypes. In addition, the extract can also inhibit the growth of human cervical carcinoma cell line, HeLa. Comet assay also confirms about the cytotoxicity compared to the control one. The whole data suggest that the RSLE could be an effective means for the biological control and can be used as pesticide.
英文关键词	Aedes aegypti Comet Assay RAPD-PCR Rhazya stricta
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000462567700004
WOS关键词	LARVICIDAL ACTIVITY ; MEDICINAL-PLANTS ; VECTOR
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333458
作者单位	[Mohammed, Baeshen N.] Univ Jeddah, Fac Sci, Dept Biol, Jeddah, Saudi Arabia; [Sanaa, Al-Attas G.; Mohamed, Ahmed M. M.; Yasir, Anwar; Abdul-Rahman, Faragalla A.; Hassan, Ramdan A.; Ayman, Elkady, I; Jazem, Mahyoub A.; Huda, Alabdali A.; Nabih, Baeshen A.] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia; [Mohamed, Ahmed M. M.] City Sci Res & Technol Applicat, Dept Nucle Acids Res, Alexandria, Egypt; [Hassan, Ramdan A.] Natl Res Ctr, Dept Cell Biol, Genet Engn & Biotechnol Div, Giza, Egypt; [Ayman, Elkady, I] Alexandria Univ, Fac Sci, Dept Zool, Alexandria, Egypt
推荐引用方式 GB/T 7714	Mohammed, Baeshen N.,Sanaa, Al-Attas G.,Mohamed, Ahmed M. M.,et al. Genotoxic effect of Rhazya stricta leaf extract against larvae of Aedes aegypti[J],2019,14(4):30-36.
APA	Mohammed, Baeshen N..,Sanaa, Al-Attas G..,Mohamed, Ahmed M. M..,Yasir, Anwar.,Abdul-Rahman, Faragalla A..,...&Nabih, Baeshen A..(2019).Genotoxic effect of Rhazya stricta leaf extract against larvae of Aedes aegypti.RESEARCH JOURNAL OF BIOTECHNOLOGY,14(4),30-36.
MLA	Mohammed, Baeshen N.,et al."Genotoxic effect of Rhazya stricta leaf extract against larvae of Aedes aegypti".RESEARCH JOURNAL OF BIOTECHNOLOGY 14.4(2019):30-36.