干旱区生态环境知识资源中心(Arid): Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute

Arid

DOI	10.18699/VJ19.456
	Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute
	Amineva, E. Yu; Gureev, A. P.; Tabatskaya, T. M.; Mashkina, O. S.; Popov, V. N.
通讯作者	Amineva, EY
来源期刊	VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
ISSN	2500-0462
EISSN	2500-3259
出版年	2019
卷号	23 期号:1 页码:15-23
英文摘要	There was an increase and intensification of droughts in connection with global climate change in recent decades. Not all plant organisms are able to adapt to changing environmental conditions. Therefore, the question of stress-resistant (drought-resistant) genotypes selection for breeding is quite urgent. This problem also concerns forest tree plants, including Scots pine, which is one of the main foresters in the Voronezh region.The results of vegetative and generative sphere analysis of individual Scotch pine trees with the help of biotechnology, molecular-genetic and cytogenetic method are given in this study. The possibility of applying the tissue culture method in vitro for testing initial plants for stress resistance, including drought resistance, is explained by the interconnection of cells, tissues and the whole plant properties. It is shown that the cytogenetic characteristics of the seed progeny and the indicators of callusogenic reactions do not always coincide in different genotypes: in some cases energy resources are spent on ontogeny protection, while, in other cases, to reproductive function maintain. There are trees, in which the state the generative sphere in arid years is at the level of optimal years, and their callus cultures reaction remains unchanged even under simulated conditions of drought. Based on the results obtained for the selection of Scots pine drought-resistant genotypes we suggest applying a system of criteria characterizing both the ability of the vegetative sphere to survive in drought conditions on the basis of the tissue culture method in vitro (callus tissue formation speed, its viability, frequency of callusogenesis), and the state of the generative sphere with the help of cytogenetic analysis of seed progeny (frequency of mitosis pathologies, the proportion of cells with micronuclei, mitotic activity). The expediency of applying the biotechnological approach was proved by analysis of the gene expression level of stress proteins: the level of AbaH gene expression correlates greatly with the indicator of the viability of callus cultures, including ones on a nutrient medium with an additional stress agent (NaCl). Trees that can be assigned to drought-resistant ones, according to the results of the analysis, should be recommended for use in breeding.
英文关键词	Pinus sylvestris L. drought drought-resistance callus gene expression viability of callus cultures mitosis
类型	Article
语种	Russian
开放获取类型	Other Gold
收录类别	ESCI
WOS记录号	WOS:000459766700003
WOS关键词	EXPRESSION ; TOLERANCE
WOS类目	Agriculture, Multidisciplinary ; Genetics & Heredity
WOS研究方向	Agriculture ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333420
作者单位	[Amineva, E. Yu; Tabatskaya, T. M.; Mashkina, O. S.; Popov, V. N.] All Russian Res Inst Forest Genet Breeding & Biot, Voronezh, Russia; [Gureev, A. P.; Mashkina, O. S.] Voronezh State Univ, Voronezh, Russia
推荐引用方式 GB/T 7714	Amineva, E. Yu,Gureev, A. P.,Tabatskaya, T. M.,et al. Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute[J],2019,23(1):15-23.
APA	Amineva, E. Yu,Gureev, A. P.,Tabatskaya, T. M.,Mashkina, O. S.,&Popov, V. N..(2019).Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute.VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII,23(1),15-23.
MLA	Amineva, E. Yu,et al."Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute".VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII 23.1(2019):15-23.