干旱区生态环境知识资源中心(Arid): Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature

Arid

DOI	10.4038/jas.v13i1.8297
	Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature
	Kim, Seon Ae; Ahn, Soon Young; Yun, Hae Keun
通讯作者	Yun, HK
来源期刊	JOURNAL OF AGRICULTURAL SCIENCES
ISSN	1391-9318
EISSN	2386-1363
出版年	2018
卷号	13 期号:1 页码:15-30
英文摘要	Purpose : The transcriptome of 'Campbell Early 'grape berries exposed to high temperature was analyzed to select useful genes by evaluating their differential expression through sequencing short reads on Solexa platform. Research Method : We assembled 118 million high-quality trimmed reads using Velvet followed by Oases. Findings : Functional categorization of expressed transcripts revealed the conservation of genes involved in various biological processes like responses to chemical (12.7%), responses to abiotic stimulus (11.8%), biosynthesis processes (11.8%), and cellular metabolic processes (10.4%) in grape berries exposed to high temperature. The major up-regulated genes included isocitratelyase, cysteine proteinases superfamily protein, cupin family protein, and glycosyl hydrolase genes, and the major down-regulated genes included flavanone 3-hydroxylase, phenylalanine ammonia lyase, chlorophyll A-B binding family protein, and polygalacturonase inhibiting protein genes in grape berries exposed to high temperature. Among genes related to grape coloration, expressions of chalcone and stilbene synthase, flavanone 3-hydroxylase, leucoanthocyanidin dioxygenase, phenylalanine ammonia lyasegenes were more strongly inhibited in berries kept at 35 degrees C than 25 degrees C. Originality/Value : Transcript sets of grape berries generated in this study will provide a resource for gene discovery and development of functional molecular markers to be used in elucidating the mechanism of tolerance to high temperature.
英文关键词	transcriptome differentially expressed genes high temperature coloration
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000483387100003
WOS关键词	HEAT-STRESS ; ARABIDOPSIS-THALIANA ; FLAVONOL SYNTHESIS ; SUNLIGHT EXPOSURE ; ACCUMULATION ; GROWTH ; BIOSYNTHESIS ; ANTHOCYANIN ; ACCLIMATION ; PROFILES
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333360
作者单位	[Kim, Seon Ae; Ahn, Soon Young; Yun, Hae Keun] Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea; [Kim, Seon Ae] RDA, Apple Res Inst, Natl Inst Hort & Herbal Sci, Gunwi 39000, South Korea
推荐引用方式 GB/T 7714	Kim, Seon Ae,Ahn, Soon Young,Yun, Hae Keun. Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature[J],2018,13(1):15-30.
APA	Kim, Seon Ae,Ahn, Soon Young,&Yun, Hae Keun.(2018).Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature.JOURNAL OF AGRICULTURAL SCIENCES,13(1),15-30.
MLA	Kim, Seon Ae,et al."Selection of Differentially Expressed Genes Using the Transcriptome Analysis of Ripening Grape Berries in Response to High Temperature".JOURNAL OF AGRICULTURAL SCIENCES 13.1(2018):15-30.