干旱区生态环境知识资源中心(Arid): Anaplasmosis in ruminants and infesting ticks vectors settling Egyptian desert: Epidemiological updates regarding genetic profiles

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	Anaplasmosis in ruminants and infesting ticks vectors settling Egyptian desert: Epidemiological updates regarding genetic profiles
	Abd El-Baky, Sayed Mohamed Mahmoud; Allam, Nesreen Allamtantawy
通讯作者	Allam, NA
来源期刊	BIOSCIENCE RESEARCH
ISSN	1811-9506
EISSN	2218-3973
出版年	2018
卷号	15 期号:3 页码:2651-2667
英文摘要	Anaplasmosis are notifiable diseases globally distributed infection due to its socioeconomic impact and the restrictions induced on life animal trading. The infection is characterized by the so called geographical isolate with zoonotic potentials. The aim of this study was determining the incidence of anaplasmosis in animal hosts with regards to geo-biotypes distribution and ixodid ticks preference in transmission into three ruminants' hosts settling 12 localities in desert provinces in Egypt. A total of 5223 adult ixodid ticks were collected from 270 ruminants; 110 camels, 120 sheep, and 40 cattle, examined seasonally from June 2014 to July 2016. The statistical analysis of infestation density of all species on each hosts was highly significant. Nucleotides similarities of ticks' 18S rDNA and 16S rDNA markers were 99-100% against Genbank records, which confirmed ticks identification by morphological key in to 4 genera: Ambylomma, Hyalomma, Boophilus, and Rhipicephalus including 14 species. Molecular screening for anaplasmosis was carried out by multi-genes sequencing technology; where 16S rDNA was the primary target. The overall Anaplasmataceae infections incidence in animals hosts and their infesting ticks' spp. were 3.753% and 47.77%, respectively. Positive PCRs were then typed into species level by three pairs of primers complementary to stretches in major surface protein 4 (msp4) and heat shock protein 60kDa (hsp60). The identities of both genes to other recorded in Genbank ranged 95-100%. The incidence of the 7 detected Anaplasma spp. in ticks recorded 36.22%, 21.94%, 11.22%, 10.72%, 7.65%, 6.63% and 5.62% by A. phagocytophilum, unclassified spp., A. ovis, A. marginale, A. plates, A. bovis, and A. central, respectively. Whereas, incidence in positive animal hosts recorded 33.33%, 29.45%, 12.40%, and 8.53%, for the unclassified spp., A. phagocytophilum, A. marginale, and A. ovis, respectively. While equal incidence recorded 5.43% was reported for A. plates, A. bovis, and A. central, respectively. Camels were found infected with all detected spp. While cattle sheep were infected except with A. ovis & A. plates in cattle, as well as, A. bovis & A. central in sheep. B. annulatus and H. dromedarii were found carriers for all detected Anaplasma spp. H. m. rufipes, on the other hand, was positive for A. phagocytophilum, A. ovis and A. plates only. The present study is the first to declare positive specimens with A. ovis, A. marginale, A. plates, and A. central not which confirmed the endemic status of anaplasmosis. Co-infections with more than one species presented alarm for possible future epidemics by new species not clearly classified which necessitate more research and should no longer be neglected.
英文关键词	Major Surface Protein 4 (msp4) Heat Shock Protein 60kDa (hsp60) Hard Ticks Anaplasmataceae
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000458382700130
WOS关键词	RHIPICEPHALUS-SANGUINEUS ; ACARI IXODIDAE ; MOLECULAR-IDENTIFICATION ; PHYLOGENETIC ANALYSIS ; MITOCHONDRIAL ; PHAGOCYTOPHILUM ; MARKERS ; CATTLE ; MARGINALE ; ANAPLASMATACEAE
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333296
作者单位	[Abd El-Baky, Sayed Mohamed Mahmoud] Desert Res Ctr, Div Anim & Poultry Prod, Dept Anim Hlth, Parasitol Unit, Cairo, Egypt; [Allam, Nesreen Allamtantawy] Natl Res Ctr, Vet Res Div, Parasitol & Anim Dis Dept, POB 12622, Cairo, Egypt
推荐引用方式 GB/T 7714	Abd El-Baky, Sayed Mohamed Mahmoud,Allam, Nesreen Allamtantawy. Anaplasmosis in ruminants and infesting ticks vectors settling Egyptian desert: Epidemiological updates regarding genetic profiles[J],2018,15(3):2651-2667.
APA	Abd El-Baky, Sayed Mohamed Mahmoud,&Allam, Nesreen Allamtantawy.(2018).Anaplasmosis in ruminants and infesting ticks vectors settling Egyptian desert: Epidemiological updates regarding genetic profiles.BIOSCIENCE RESEARCH,15(3),2651-2667.
MLA	Abd El-Baky, Sayed Mohamed Mahmoud,et al."Anaplasmosis in ruminants and infesting ticks vectors settling Egyptian desert: Epidemiological updates regarding genetic profiles".BIOSCIENCE RESEARCH 15.3(2018):2651-2667.