干旱区生态环境知识资源中心(Arid): Isolation and Characterisation of a Soil Microbial Streptomyces Strain that Produces Membrane-Active Antimicrobial Metabolites

Arid

DOI	10.9734/JPRI/2018/39144
	Isolation and Characterisation of a Soil Microbial Streptomyces Strain that Produces Membrane-Active Antimicrobial Metabolites
	Azerang, Parisa; Owlia, Parviz; Khalaj, Vahid; Kobarfard, Farzad; Sardari, Soroush
通讯作者	Sardari, S
来源期刊	JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL
ISSN	2456-9119
出版年	2018
卷号	21 期号:6
英文摘要	In continuation of our previous research, more than 60 microbial strains that were isolated from soil samples collected from desert, farming and mineral regions of Iran and sixteen isolated strains that produced antimicrobial metabolites were found to be effective against Escherichia coli, Candida albicans and Saccharomyces cerevisiae and Staphylococcus aureus microorganisms. A total of 3 out of 16 strains produced membrane-active ethyl acetate fractions based on artificial vesicle assay. The antimicrobial and membrane interfering activities of crude extract and fractions from Actinomycetes strain were investigated in the current study. We report the isolation two compounds from biologically active fractions obtained from Streptomyces sp 0811 culture. Fractionation of Streptomyces sp 0811 demonstrated antimicrobial activities against bacteria and yeast strains. Vesicle assay showed membrane activity for the mentioned fraction. This suggests the presence of an antimicrobial membrane active agent. Further fractionation and purification strategies were applied to finally identify the isolated moleculesby using appropriate spectroscopic methods, such as (HNMR)-H-1, COSY, and LC-MS. The two identified structures are described to be indole-type and ether amino derivative.
英文关键词	Drug development membrane active ethyl acetate fraction actinomycetes antimicrobial
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000454621700005
WOS关键词	ENDOPHYTES ; DISCOVERY ; FUNGI ; ASSAM
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333173
作者单位	[Azerang, Parisa; Sardari, Soroush] Pasteur Inst Iran, Biotechnol Res Ctr, Dept Med Biotechnol, Drug Design & Bioinformat Unit, Tehran, Iran; [Owlia, Parviz] Shahed Univ, Fac Med, Mol Microbiol Res Ctr, Tehran, Iran; [Khalaj, Vahid] Pasteur Inst Iran, Biotechnol Res Ctr, Dept Med Biotechnol, Tehran, Iran; [Kobarfard, Farzad] Shahid Beheshti Univ Med Sci, Sch Pharm, Dept Med Chem, Tehran, Iran
推荐引用方式 GB/T 7714	Azerang, Parisa,Owlia, Parviz,Khalaj, Vahid,et al. Isolation and Characterisation of a Soil Microbial Streptomyces Strain that Produces Membrane-Active Antimicrobial Metabolites[J],2018,21(6).
APA	Azerang, Parisa,Owlia, Parviz,Khalaj, Vahid,Kobarfard, Farzad,&Sardari, Soroush.(2018).Isolation and Characterisation of a Soil Microbial Streptomyces Strain that Produces Membrane-Active Antimicrobial Metabolites.JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL,21(6).
MLA	Azerang, Parisa,et al."Isolation and Characterisation of a Soil Microbial Streptomyces Strain that Produces Membrane-Active Antimicrobial Metabolites".JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL 21.6(2018).