干旱区生态环境知识资源中心(Arid): In-silico identification and validation of miRNAs in pearl millet [Pennisetum glaucum L.]

Arid

DOI	10.1016/j.cpb.2018.09.007
	In-silico identification and validation of miRNAs in pearl millet [Pennisetum glaucum L.]
	Kumar, Amit; Kumar, Sushil; Fougat, Ranbir S.; Zala, Harshvardhan N.
通讯作者	Kumar, A
来源期刊	CURRENT PLANT BIOLOGY
EISSN	2214-6628
出版年	2018
卷号	14 页码:41-49
英文摘要	Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important C-4 crop in the arid and semi-arid regions of Asian and African countries which is under cultivation for grain, forage and stover. Here, we report identification of the conserved miRNAs in pearl millet by mining publicly available data sets including expressed sequence tag (EST) sequences, genome survey sequences (GSS), and nucleotide sequences. Homology search of 7240 known mature miRNAs of plant species against 6014 Unigene sequences of pearl millet resulted in identification of 14 potential miRNAs belonging to 11 different families. The length of predicated precursor and mature miRNAs ranged from 60 to 109 nucleotides and 18-23 nucleotides, respectively. The negative minimal folding free energy of precursor miRNAs varied significantly between - 13 to - 41.73 kcal/mol. Five out of 14 predicted miRNAs were validated in root and leaf/shoot tissues of seedlings of two pearl millet genotypes (J2290 and 7042S) using quantitative real time polymerase chain reaction (qRT-PCR). The psRNATarget server predicted 33 potential target genes and their probable functions. Most of the pearl millet miRNA target genes were involved in stress response, transcriptional regulation, signal transduction, DNA replication proteins, male sterility, metabolic enzymes, plant growth and development. The results of the present study will help in the understanding of mechanism of miRNA functioning in pearl millet.
英文关键词	Expression microRNA Pearl millet RT-PCR
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000451550200005
WOS关键词	PLANT MICRORNA ; COMPUTATIONAL IDENTIFICATION ; GENE-EXPRESSION ; BIOGENESIS ; TARGETS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333095
作者单位	[Kumar, Amit; Kumar, Sushil; Fougat, Ranbir S.; Zala, Harshvardhan N.] Anand Agr Univ, Dept Agr Biotechnol, Anand 388110, Gujarat, India
推荐引用方式 GB/T 7714	Kumar, Amit,Kumar, Sushil,Fougat, Ranbir S.,et al. In-silico identification and validation of miRNAs in pearl millet [Pennisetum glaucum L.][J],2018,14:41-49.
APA	Kumar, Amit,Kumar, Sushil,Fougat, Ranbir S.,&Zala, Harshvardhan N..(2018).In-silico identification and validation of miRNAs in pearl millet [Pennisetum glaucum L.].CURRENT PLANT BIOLOGY,14,41-49.
MLA	Kumar, Amit,et al."In-silico identification and validation of miRNAs in pearl millet [Pennisetum glaucum L.]".CURRENT PLANT BIOLOGY 14(2018):41-49.