干旱区生态环境知识资源中心(Arid): DETERMINATION OF PALBOCICLIB IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Arid

DOI	10.13040/IJPSR.0975-8232.9(9).3883-88
	DETERMINATION OF PALBOCICLIB IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - ULTRAVIOLET DETECTION
	Nalanda, R. B.; Rao, A. Srinivasa; Sankar, D. Gowri
通讯作者	Rao, AS
来源期刊	INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH
ISSN	0975-8232
出版年	2018
卷号	9 期号:9 页码:3883-3888
英文摘要	A simple, sensitive, and high-performance liquid chromatography ultraviolet detection (HPLC-UV) method was developed and validated for the quantification of palbociclib in human plasma. Plasma samples were processed by solid phase extraction using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Commercial imatinib was used as an internal standard. Palbociclib and imatinib (IS) in human plasma were analyzed using a mobile phase of acetonitrile and 0.1% Triethylamine TEA (pH 3.3; adjusted with 50% Ortho-phosphoric acid) (70:30, v/v), on a Agilent Zorbax C18 column (150 x 4.6 mm i.d., 5 mu m) using isocratic elution at a flow rate of 1.0 mL/min with ultraviolet detection at 266 nm. The method was validated over a concentration range of 0.1-3.0 mu g/mL (r(2) >= 0.998) with coefficient of variation for intraday precision of 3.92%, 3.56% and 2.28% for 0.5. 1.5, and 2.5 mu g/mL respectively. The lower limit of detection was 50 ng/mL. The extraction recovery rates for palbociclib ranged from 66.69% to 80.97%. The infra- and inter-day precision was below 5.0%, and the accuracy ranged from 89.40 - 112% over the linear range. No notable matrix effects were observed. The proposed RP-HPLC method was successfully applicable for clinical therapeutic drug monitoring programs and found suitable application in design pharmacokinetic studies.
英文关键词	Palbociclib Plasma Solid phase extraction HPLC
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	ESCI
WOS记录号	WOS:000445868400036
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333004
作者单位	[Nalanda, R. B.; Rao, A. Srinivasa] Shri Vishnu Coll Pharm, Dept Pharmaceut Anal & Qual Assurance, West Godavari 534202, Andhra Prades, India; [Sankar, D. Gowri] Andhra Univ, Dept Pharmaceut Anal & Qual Assurance, Coll Pharmaceut Sci, Visakhapatnam 530003, Andhra Prades, India
推荐引用方式 GB/T 7714	Nalanda, R. B.,Rao, A. Srinivasa,Sankar, D. Gowri. DETERMINATION OF PALBOCICLIB IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - ULTRAVIOLET DETECTION[J],2018,9(9):3883-3888.
APA	Nalanda, R. B.,Rao, A. Srinivasa,&Sankar, D. Gowri.(2018).DETERMINATION OF PALBOCICLIB IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - ULTRAVIOLET DETECTION.INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH,9(9),3883-3888.
MLA	Nalanda, R. B.,et al."DETERMINATION OF PALBOCICLIB IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - ULTRAVIOLET DETECTION".INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH 9.9(2018):3883-3888.