干旱区生态环境知识资源中心(Arid): Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency

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DOI	10.1016/j.bcab.2018.04.004
	Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency
	Abd El Aty, Abeer A.; Shehata, Abeer N.; Shaheen, Tharwat I.
通讯作者	Abd El Aty, AA
来源期刊	BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
ISSN	1878-8181
出版年	2018
卷号	14 页码:375-385
英文摘要	In our search for a pullulanase production from unconventional sources, Bacillus subtilis MF467279 was isolated from the desert plant species Salsola imbricate, that collected from Tur Sinai, Egypt. The optimization of pullulanase enzyme was performed in three stages. First, the two level Plackets-Burman design to screen the medium constituents that significantly affect the enzyme production. Secondly experiment was important for optimization of the most effective constituents using central composite design (CCD). In the third experiment Two-factor-five-level central composite design (CCD) was applied to identify the best initial pH and agitation (rpm) conditions. A maximal enzyme activity of 75.13 U/ml was more than 4.53-folds the activity obtained using the basal medium. Application of partially purified pullulanase enzyme signified that, the fraction contained mixture of pullulanase and amylase was effectively improved the desizing process of fabrics even at lowest dose as indicated from violet scale scale (TEGEWA), weight loss and Scanning Electron Microscopy (SEM).
英文关键词	Pullulanase Bacillus subtilis MF467279 Statistical designs Optimization Desizing
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000432771500047
WOS关键词	GROWTH-PROMOTING RHIZOBACTERIA ; THERMOSTABLE PULLULANASE ; ALPHA-AMYLASE
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332789
作者单位	[Abd El Aty, Abeer A.] Natl Res Ctr Dokki, Chem Nat & Microbial Prod Dept, Giza, Egypt; [Abd El Aty, Abeer A.] Hafr Al Batin Univ, Fac Educ, Biol Dept, Al Batin, Saudi Arabia; [Shehata, Abeer N.] Natl Res Ctr, Biochem Dept, Giza, Egypt; [Shaheen, Tharwat I.] Natl Res Ctr, Text Res Div, Giza, Egypt
推荐引用方式 GB/T 7714	Abd El Aty, Abeer A.,Shehata, Abeer N.,Shaheen, Tharwat I.. Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency[J],2018,14:375-385.
APA	Abd El Aty, Abeer A.,Shehata, Abeer N.,&Shaheen, Tharwat I..(2018).Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency.BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY,14,375-385.
MLA	Abd El Aty, Abeer A.,et al."Production and sequential optimization of Bacillus subtilis MF467279 pullulanase by statistical experimental designs and evaluation of its desizing efficiency".BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 14(2018):375-385.