干旱区生态环境知识资源中心(Arid): Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

Arid

DOI	10.4238/gmr16039866
	Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers
	Costa, J. C.; Fracetto, G. G. M.; Fracetto, F. J. C.; Souza, T. C.; Santos, M. V. F.; Lira Junior, M. A.
通讯作者	Lira, MA
来源期刊	GENETICS AND MOLECULAR RESEARCH
ISSN	1676-5680
出版年	2018
卷号	17 期号:1
英文摘要	Genetic diversity is the basis for genetic improvement, providing genes for new cultivars. Stylosanthes scabra is a legume used in pastures, presenting economic importance in tropical and subtropical regions. Molecular markers are targeted in population studies, thus we evaluated the genetic diversity distribution of S. scabra in Brazilian semi-arid northeastern among and within populations, from molecular markers ISSR. Four natural populations of S. scabra located in the municipalities of Santa Cruz do Capibaribe, Floresta, Sertania and Petrolina from Pernambuco state were used. We selected seven ISSR primers for amplification and analyzed 75 individuals. A total of 88 bands were amplified with a percentage of 95.27% polymorphism at the species level. AMOVA test revealed that 40.0% of the total genetic variation is within populations while 60.0% among populations. The population differentiation was 0.332 and the migrant's number per generation was 0.5. Grouping analysis confirmed a high level of differentiation among populations. The populations with the greatest variability were Santa Cruz do Capibaribe and Petrolina, and the ISSR markers were efficient for the genetic diversity quantification in S. scabra, which presented greater variability among populations than within populations. In short, this variability will be very useful in future breeding programs for this species.
英文关键词	Molecular markers Legumes Variability Breeding programs
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000426346200001
WOS关键词	RAPD MARKERS ; VARIABILITY ; ACCESSIONS ; CAPITATA ; FABACEAE ; SIZE ; MACROCEPHALA ; SOFTWARE ; UPDATE ; PLANTS
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332714
作者单位	[Costa, J. C.] Inst Fed Pernambuco, Vitoria Santo Antao, PE, Brazil; [Fracetto, G. G. M.; Fracetto, F. J. C.; Lira Junior, M. A.] Univ Fed Rural Pernambuco, Dept Agron, Recife, PE, Brazil; [Souza, T. C.; Santos, M. V. F.] Univ Fed Rural Pernambuco, Dept Zootecn, Recife, PE, Brazil
推荐引用方式 GB/T 7714	Costa, J. C.,Fracetto, G. G. M.,Fracetto, F. J. C.,et al. Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers[J],2018,17(1).
APA	Costa, J. C.,Fracetto, G. G. M.,Fracetto, F. J. C.,Souza, T. C.,Santos, M. V. F.,&Lira Junior, M. A..(2018).Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers.GENETICS AND MOLECULAR RESEARCH,17(1).
MLA	Costa, J. C.,et al."Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers".GENETICS AND MOLECULAR RESEARCH 17.1(2018).