干旱区生态环境知识资源中心(Arid): Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia

Arid

DOI	10.4103/pr.pr_38_17
	Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia
	Molina-Romo, Edna D.; Garibay-Escobar, Adriana; Valenzuela-Antelo, Olivia; Ruiz-Bustos, Eduardo; Hernandez Martinez, Javier; Velazquez, Carlos; Rascon-Valenzuela, Luisa A.; Enrique Robles-Zepeda, Ramon
通讯作者	Robles-Zepeda, RE
来源期刊	PHARMACOGNOSY RESEARCH
ISSN	0974-8490
EISSN	0976-4836
出版年	2018
卷号	10 期号:1 页码:55-59
英文摘要	Background: Ziziphus obtusifolia is a spiny shrub found in Northwest Mexico desert, with traditional medicinal use to treat several diseases including cancer. Objective: The aims of the present study were to evaluate the antiproliferative and apoptotic activities of the aerial parts of this plant. Materials and Methods: The methanol extract and its fractions were prepared using several solvents. The antiproliferative activity was evaluated by the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium) (MTT) assay on HeLa, A549, RAW 264.7, M12.C3.F6, and L-929 cell lines, and the apoptotic activity using Annexin V and (5,50,6,60-tetra-chloro-1,10,3,30-tetra-ethylbenzimidazol-carbocyanine iodide) staining. The most active fraction was further separated by column chromatography. Results: The most active fraction was hexane with an IC50 of 90.4 mu g/mL against RAW 264.7, 94 mu g/mL against M12. C3. F6, 165.5 mu g/mL against HeLa and 187.7 mu g/mL against A549 cell lines. In apoptotic activity assays the methanol extract and its n-hexane fraction were found to induce mitochondrial depolarization in HeLa cells (83 and 87% respectively), and both induced the externalization of the phosphatidylserine increasing the percentage of cells in early apoptosis from 1.4% in untreated control cells, to 1.9% and 3.5% for methanol extract and n-hexane fraction-treated cells, respectively, statistically different for the total percentage of apoptotic cells (P < 0.05). Conclusions: These results show that Z. obtusifolia has antiproliferative and apoptotic activities in vitro and confirms its use in traditional medicine.
英文关键词	Antiproliferative activity apoptotic activity Ziziphus obtusifolia
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000425976000009
WOS关键词	CELL APOPTOSIS ; CANCER-THERAPY ; JUJUBE ; PATHWAY ; FRUIT
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332710
作者单位	[Molina-Romo, Edna D.; Garibay-Escobar, Adriana; Valenzuela-Antelo, Olivia; Ruiz-Bustos, Eduardo; Velazquez, Carlos; Rascon-Valenzuela, Luisa A.; Enrique Robles-Zepeda, Ramon] Univ Sonora, Dept Chem Biol Sci, Blvd Luis Encinas & Rosales S-N, Hermosillo 83000, Sonora, Mexico; [Hernandez Martinez, Javier] Veracruzana Univ, Unit Support Serv Analitycal Resolut, Xico, Veracruz, Mexico
推荐引用方式 GB/T 7714	Molina-Romo, Edna D.,Garibay-Escobar, Adriana,Valenzuela-Antelo, Olivia,et al. Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia[J],2018,10(1):55-59.
APA	Molina-Romo, Edna D..,Garibay-Escobar, Adriana.,Valenzuela-Antelo, Olivia.,Ruiz-Bustos, Eduardo.,Hernandez Martinez, Javier.,...&Enrique Robles-Zepeda, Ramon.(2018).Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia.PHARMACOGNOSY RESEARCH,10(1),55-59.
MLA	Molina-Romo, Edna D.,et al."Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia".PHARMACOGNOSY RESEARCH 10.1(2018):55-59.