Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.14719/pst.2017.4.1.271 |
| In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan | |
| Parihar, Suman | |
| 通讯作者 | Parihar, S |
| 来源期刊 | PLANT SCIENCE TODAY
 |
| ISSN | 2348-1900 |
| 出版年 | 2017 |
| 卷号 | 4期号:1页码:21-26 |
| 英文摘要 | In vitro regeneration protocol has been standardized for highly medicinal and threatened succulent Ceropegia bulbosa Roxb. The paper focuses on morphogenic response of nodal explant when cultured on MS media. Murashige and Skoog medium supplemented with 6-benzyladenine (BA) (2.0 mgl(-1)) was found optimum for axillary shoot bud induction with 83.4 % response. Further shoots were multiplied through repetitive (3-4 times) transfer of the original explant and by subculture of the in vitro generated shoots. Maximum number of shoots 5.7 +/- 0.78 with shoot length of 3.6 +/- 0.82 cm was achieved on MS medium augmented with combination of 0.25 mgl(-1) BA + 0.25 mgl(-1) KN + 0.1 mgl(-1) IAA and additives (50.0 mgl(-1) ascorbic acid, 25 mgl(-1) each of citric acid, arginine and adenine sulphate). For ex vitro rooting, pulse treatment of IBA 250 mgl(-1) for 3 min was found optimum. The rooted shoots were successfully hardened in the green house condition (RH 75-80% at 26-28 degrees C) and about 80 % shoots were transferred to the garden. |
| 英文关键词 | Ceropegia bulbosa khedula herbal medicine ex vitro root |
| 类型 | Article |
| 语种 | 英语 |
| 开放获取类型 | Other Gold, Green Published |
| 收录类别 | ESCI |
| WOS记录号 | WOS:000449591800003 |
| WOS关键词 | ANETHUM-GRAVEOLENS ; PHARMACEUTICALLY IMPORTANT ; SOMATIC EMBRYOGENESIS ; TISSUE-CULTURES ; CLONAL FIDELITY ; RAPD ANALYSIS ; THAR DESERT ; ORGANOGENESIS ; REGENERATION ; PROPAGATION |
| WOS类目 | Plant Sciences |
| WOS研究方向 | Plant Sciences |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/332619 |
| 作者单位 | [Parihar, Suman] Jai Narain Vyas Univ, Ctr Adv Study, Dept Bot, Jodhpur 342001, Rajasthan, India |
| 推荐引用方式 GB/T 7714 | Parihar, Suman. In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan[J],2017,4(1):21-26. |
| APA | Parihar, Suman.(2017).In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan.PLANT SCIENCE TODAY,4(1),21-26. |
| MLA | Parihar, Suman."In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan".PLANT SCIENCE TODAY 4.1(2017):21-26. |
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