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	Screening the Egyptian actinomycetes as candidates for anti-microbial compounds and identification of Nocardiopsis dassonvillei HBUM17483
	Abed, Nermine Nasr Eldin
通讯作者	Abed, NNE
来源期刊	BIOSCIENCE RESEARCH
ISSN	1811-9506
EISSN	2218-3973
出版年	2017
卷号	14 期号:2 页码:427-445
英文摘要	The need for new antibiotics is increasing with the increase in antibiotics resistance. The present work focused on the isolation of antibiotic producing actinomycetes from sandy soil. Twenty-four actinomycetes isolates were isolated from sandy soil collected from different locations of Cairo- Egypt. The isolates were tested for their anti-microbial activity, one isolate showed good results against Gram (+ve), Bacillus subtilis,Staphylococcus aureus and Streptococcus pyogenes and Gram (-ve) Pseudomonas aeruginosa,Klebsiella pneumonia and Campylobacter jejuni, no antimicrobial activity was observed against filamentous fungi like Aspergillus fumigates, Syncephalastrumracemosum, Geotricumcandidum and unicellular fungi Candida albicans. Morphological,physiological and phylogenetic analysis (16S r RNA); in addition to biochemical studies and cultural characteristics that described by the International Keys of Bergey's Manual for identification of actinomycetes as well as chemical analysis of the cell wall,were carried out for the active isolate under study, which proved that a 99% similarity with Nocardiopsis dassonvillei strain HBUM174832. The active metabolites were extracted by using ethyl acetate as a solvent. Compounds No. 10 and No. 11 were the most active fractions against G-ve bacteria and G+ve bacteria. The separation, purification and characterization of the active metabolites were performed using Column & thin layer chromatography. The physicochemical studies of the purified active metabolites (10&11) have been investigated. The two purified metabolites (Compounds10&11) were found to belonging to 1, 3-2-hydroxyethyl, phenyl, prop an-2-one with M.W=178M(+) and emperical formula C11H14O2 and 2- hydroxyl-1-,3-,2- hydroxy-3-oxobutyl phenyl) hexan-3-one with M.W.= 279M(+)-1and empirical formula C16H22O4 respectively. The MICs values were determined and pre-luminary cytotoxicity were also assessed. It was concluded that Actinomycete isolate NO.A 18, identified as Nocardiopsis dassonvillei strain HBUM174832 has antibiotic producing potential that could be used in the development of new antibiotics for pharmaceutical applications.
英文关键词	Actinomycetes Antagonistic Activity Antimicrobial Activity Agar well diffusion method concentration Separation Purification Characterization Nocardiopsis sp.
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000447365800036
WOS关键词	PHYLOGENETIC ANALYSIS ; SELECTIVE ISOLATION ; BACTERIA ; DIVERSITY ; DESERT ; STRAIN ; SOILS ; NOV.
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332613
作者单位	[Abed, Nermine Nasr Eldin] Al Azhar Univ, Fac Sci For Girls, Dept Bot & Microbiol, Cairo, Egypt
推荐引用方式 GB/T 7714	Abed, Nermine Nasr Eldin. Screening the Egyptian actinomycetes as candidates for anti-microbial compounds and identification of Nocardiopsis dassonvillei HBUM17483[J],2017,14(2):427-445.
APA	Abed, Nermine Nasr Eldin.(2017).Screening the Egyptian actinomycetes as candidates for anti-microbial compounds and identification of Nocardiopsis dassonvillei HBUM17483.BIOSCIENCE RESEARCH,14(2),427-445.
MLA	Abed, Nermine Nasr Eldin."Screening the Egyptian actinomycetes as candidates for anti-microbial compounds and identification of Nocardiopsis dassonvillei HBUM17483".BIOSCIENCE RESEARCH 14.2(2017):427-445.

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