干旱区生态环境知识资源中心(Arid): LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples

Arid

DOI	10.13005/ojc/330539
	LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples
	Reddy, B. Sidda; Sudhakar, Y.; Rao, Y. Subba; Prasad, P. Reddy; Sreedhar, N. Y.
通讯作者	Sreedhar, NY
来源期刊	ORIENTAL JOURNAL OF CHEMISTRY
ISSN	0970-020X
EISSN	2231-5039
出版年	2017
卷号	33 期号:5 页码:2459-2469
英文摘要	A modest liquid chromatography/tandem mass spectrometric method was developed and validated to determine three tetracycline antibiotics and their epimers tetracycline, oxytetracycline, chlortetracycline, 4-epi tetracycline, 4-epi oxytetracycline, 4-epi chlortetracycline in shrimp samples respectively. The procedure was involved the extraction of tetracyclines and its epimers from homogenized sample with EDTA-McIlvaine buffer followed by solid phase extraction clean up with Oasis HLB cartridge. The analysis was carried out using Inertsil ODS-3V, 5 micron, 150x4.6 mm column and mobile phase consists of 5 mM Oxalic acid in water (A) and 0.1% formic acid in methanol (B) in gradient mode at a flow rate of 0.8 mL/minutes. The final extracts were analyzed by the sensitive and selective LC/ESI/MS/MS operating in positive ion multiple reaction monitoring (MRM) mode.
英文关键词	Tectracylines Antibiotics Shrimp Liquid chromatography Tandem mass spectrometry
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	ESCI
WOS记录号	WOS:000428327100039
WOS关键词	TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLUORESCENCE DETECTION ; OXYTETRACYCLINE ; ANTIBIOTICS ; RESIDUES ; MUSCLE ; MILK ; TISSUES ; HPLC
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332568
作者单位	[Reddy, B. Sidda; Prasad, P. Reddy; Sreedhar, N. Y.] SV Univ, Dept Chem, Tirupati 517502, AP, India; [Reddy, B. Sidda; Sudhakar, Y.] First Source Lab Solut LLP Analyt, Hyderabad, TS, India; [Rao, Y. Subba] SV Univ, DST PURSE Ctr, Tirupati 517502, AP, India
推荐引用方式 GB/T 7714	Reddy, B. Sidda,Sudhakar, Y.,Rao, Y. Subba,et al. LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples[J],2017,33(5):2459-2469.
APA	Reddy, B. Sidda,Sudhakar, Y.,Rao, Y. Subba,Prasad, P. Reddy,&Sreedhar, N. Y..(2017).LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples.ORIENTAL JOURNAL OF CHEMISTRY,33(5),2459-2469.
MLA	Reddy, B. Sidda,et al."LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples".ORIENTAL JOURNAL OF CHEMISTRY 33.5(2017):2459-2469.