干旱区生态环境知识资源中心(Arid): Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers

Arid

DOI	10.1016/j.mgene.2017.09.002
	Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers
	Kumari, Suman; Yadav, Kuldeep; Singh, Narender
通讯作者	Singh, N
来源期刊	META GENE
ISSN	2214-5400
出版年	2017
卷号	14 页码:129-133
英文摘要	Salvadora persica L. (family Salvadoraceae) is a highly threatened medicinally and economically important arid zone plant species. With ever increasing demand, there is a pressing need for conservation and mass multiplication of this plant through biotechnological approaches. A large number of complete plantlets were obtained by direct regeneration from the nodal explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l 6-benzylaminopurine (BAP) + 1.0 mg/l indole-3-acetic acid (IAA) containing 30 g/l sucrose and 8 g/l agar at 16 h photoperiod. A high frequency of in vitro rooting was obtained on half strength MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). The micropropagated plantlets were hardened using sterilized soil and sand mixture (3:1) and successfully transferred under field conditions. The genetic stability of micropropagated clones was evaluated using random amplified polymorphic DNA (RAPD) analysis. During the study a total of 43 RAPD primers were screened, out of which 8 primers produced a total of 37 clear, distinct and reproducible amplicons. The amplified products of all the regenerated plants were monomorphic and were similar to the mother plant, thus confirming the genetic homogeneity of tissue culture raised plants. To the best of our knowledge, it is the first report on the assessment of the genetic fidelity in micropropagated plants of S. persica, which could be successfully applied as one of the safest modes for the production of true-to-type plants for the germplasm conservation.
英文关键词	Salvadora persica Threatened medicinal tree Genetic fidelity RAPD
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000419719700023
WOS关键词	ISSR MARKERS ; TISSUE CULTURES ; STABILITY ; RAPD ; PAKISTAN ; GROWTH ; SINDH
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332478
作者单位	[Kumari, Suman; Yadav, Kuldeep; Singh, Narender] Kurukshetra Univ, Dept Bot, Haryana 136119, India
推荐引用方式 GB/T 7714	Kumari, Suman,Yadav, Kuldeep,Singh, Narender. Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers[J],2017,14:129-133.
APA	Kumari, Suman,Yadav, Kuldeep,&Singh, Narender.(2017).Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers.META GENE,14,129-133.
MLA	Kumari, Suman,et al."Evaluation of genetic fidelity among micropropagated plants of Salvadora persica L. using DNA-based markers".META GENE 14(2017):129-133.