干旱区生态环境知识资源中心(Arid): Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction

Arid

DOI	10.3724/SP.J.1123.2017.04033
	Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction
	Zhou Yuhui; Zeng Zhenling; Yu Jingxian; Liu Rong; Pan Lihua; Zhong Qiuyu; Chen Hong
通讯作者	Chen, H
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2017
卷号	35 期号:9 页码:995-1002
英文摘要	A method for the simultaneous determination of trypanocidal diminazene aceturate (DIM) and isometamidium chloride (ISM) that containing benzamidine groups in cattle tissues was developed by high performance liquid chromatography (HPLC) with solid. phase extraction (SPE). The tissue samples were extracted with different proportions of water. acetonitrile, then were cleaned up by Oasis WCX cartridges. DIM and ISM were separated by HPLC with a Spherisorb CN column (250 mmx4.6 mm, 5 mu m). Acetonitrile. 0.05 mol/L ammonium formate solution (pH 2.4) was used as mobile phases with gradient elution. The detection wavelength of UV was set at 380 nm. The limits of detection (LODs) and the limits of quantification (LOQs) of DIM and ISM in cattle tissues were 0.01 mgkg and 0.025 mg/kg, respectively. The correlation coefficients (r) of DIM and ISM in cattle tissues were not less than 0.999 3. The average recoveries of DIM and ISM at three spiked levels were 82.2% - 97.6% with the intra-day relative standard derivations (RSDs) of 0.3% - 5.2% (n = 5) and inter. day RSDs of 1.3% - 5.2% (n = 15). The method was successfully applied to the analysis of DIM and ISM in cattle tissues. The method is rapid, sensitive and repeatable for the determination of diminazene aceturate and isometamidium chloride in cattle tissues.
英文关键词	high performance liquid chromatography (HPLC) solid-phase extraction (SPE) multi-residue determination diminazene aceturate (DIM) isometamidium chloride (ISM) cattle tissues
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000419489300013
WOS关键词	TRYPANOSOMA-CONGOLENSE ; HPLC ; DIACETURATE ; PHARMACOKINETICS ; SUSCEPTIBILITY ; IMPURITIES ; PLASMA ; MILK
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332469
作者单位	[Zhou Yuhui; Zeng Zhenling; Yu Jingxian; Liu Rong; Pan Lihua; Zhong Qiuyu; Chen Hong] South China Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues, Guangzhou 510642, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhou Yuhui,Zeng Zhenling,Yu Jingxian,et al. Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction[J],2017,35(9):995-1002.
APA	Zhou Yuhui.,Zeng Zhenling.,Yu Jingxian.,Liu Rong.,Pan Lihua.,...&Chen Hong.(2017).Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction.CHINESE JOURNAL OF CHROMATOGRAPHY,35(9),995-1002.
MLA	Zhou Yuhui,et al."Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction".CHINESE JOURNAL OF CHROMATOGRAPHY 35.9(2017):995-1002.