干旱区生态环境知识资源中心(Arid): Rapid and simultaneous determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry coupled with pass-through solid phase extraction

Arid

DOI	10.3724/SP.J.1123.2017.04018
	Rapid and simultaneous determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry coupled with pass-through solid phase extraction
	Li Shiyan; Wang Yang; Wang Dingnan; Wu Hongxi; Ding Xueyan; Cui Yiwei; Shen Qing
通讯作者	Wang, Y
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2017
卷号	35 期号:8 页码:794-800
英文摘要	An analytical method was developed for the simultaneous and rapid determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with pass-through solid phase extraction (SPE). The samples were extracted with methanol-water (90:10, v/v) after heat treatment by water bath at 80., and then cleaned up with an Oasis PRiME HLB pass-through SPE column. The samples were analyzed directly on a Waters XSelect HSS T3 column using 0.1% (v/v) aqueous formic acid and acidified acetonitrile (0.1% formic acid, v/v) as mobile phases. Qualitative and quantitative analysis of the analytes was carried out under the multiple reaction monitoring mode with positive electrospray ionization. The matrix matching external standard method was used for quantitation analysis. To solve the problem of parent ion selection of the microcystins, the ionization characteristics of microcystins were evaluated under different mobile phase conditions. Finally, the results showed that the acid could promote the intensity of the doubly charged ions significantly. The calibration curves were linear well in the corresponding concentration ranges, with correlation coefficient >= 0.99. The limits of quantification ranged from 0.30 to 2.0 mu g/kg. The average spikedrecoveries for the seven microcystins were between 70.6% and 96.1% with the relative standard deviations of 3..4%-9.6%. The proposed method is sensitive, accurate, and efficient. It is applicable for the determination of microcystins in fish meat.
英文关键词	pass-through solid-phase extraction liquid chromatography-tandem mass (LC-MS/MS) doubly charged ions microcystin (MC) fish meat
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000411258200002
WOS关键词	CYANOBACTERIA ; PROGRESS ; WATERS ; LIVER
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332274
作者单位	[Li Shiyan; Wang Yang; Wang Dingnan; Wu Hongxi; Ding Xueyan] Aquat Prod Qual Inspect Ctr Zhejiang Prov, Hangzhou 310023, Zhejiang, Peoples R China; [Li Shiyan; Cui Yiwei; Shen Qing] Zhejiang Gongshang Univ, Inst Seafood, Hangzhou 310012, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Li Shiyan,Wang Yang,Wang Dingnan,et al. Rapid and simultaneous determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry coupled with pass-through solid phase extraction[J],2017,35(8):794-800.
APA	Li Shiyan.,Wang Yang.,Wang Dingnan.,Wu Hongxi.,Ding Xueyan.,...&Shen Qing.(2017).Rapid and simultaneous determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry coupled with pass-through solid phase extraction.CHINESE JOURNAL OF CHROMATOGRAPHY,35(8),794-800.
MLA	Li Shiyan,et al."Rapid and simultaneous determination of seven microcystins in fish meat by liquid chromatography-tandem mass spectrometry coupled with pass-through solid phase extraction".CHINESE JOURNAL OF CHROMATOGRAPHY 35.8(2017):794-800.