干旱区生态环境知识资源中心(Arid): Red-legged partridge (Alectoris rufa) de-novo transcriptome assembly and identification of gene-related markers

Arid

DOI	10.1016/j.gdata.2017.02.003
	Red-legged partridge (Alectoris rufa) de-novo transcriptome assembly and identification of gene-related markers
	Sevane, Natalia; Canon, Javier; Eusebi, Paulina G.; Gil, Ignacio; Dunner, Susana
通讯作者	Sevane, N
来源期刊	GENOMICS DATA
ISSN	2213-5960
出版年	2017
卷号	11 页码:132-134
英文摘要	The red-legged partridge (Alectoris rufa) has a great socio-economic importance as a game species and is reared by millions in farms in several European countries. The ability to respond to a wide spectrum of pathogens and environmental changes is key for farm-reared animals that, as such, face even higher pathogen exposure and specifically for those submitted to restocking programs. In this study, RNA-sequencing and de-novo assembly of genes expressed in different immune tissues were performed. The raw FASTQ files were submitted to the NCBI SRA database with accession number PRJNA289204. A total of 94.2 million reads were obtained and assembled into 51,403 contigs using OASES software. The final annotated partridge immune transcriptome comprises almost 7000 unigenes, available as FASTA in the supplementary material. A total of 12,828 microsatellites and 33,857 Single Nucleotide Polymorphisms (SNPs) were identified. The candidate gene sequences and the large number of potential genetic markers from the red-legged partridge transcriptome reliably identified through the use for the first time of a high coverage 100-bp paired-end RNA-seq protocol, provide new tools for future studies in this and related species, thus contributing to the ongoing development of genomic resources in avian species. Further investigation into candidate genes and gene-associatedmarkers will help to uncover individual variability in the resistance to infections and other external aggressions in partridges. (C) 2017 The Authors. Published by Elsevier Inc.
英文关键词	RNA-seq Transcriptomic signatures Immune response SNPs Microsatellites
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000395325800035
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/332074
作者单位	[Sevane, Natalia; Canon, Javier; Eusebi, Paulina G.; Gil, Ignacio; Dunner, Susana] Univ Complutense, Fac Vet, Dept Anim Prod, E-28040 Madrid, Spain
推荐引用方式 GB/T 7714	Sevane, Natalia,Canon, Javier,Eusebi, Paulina G.,et al. Red-legged partridge (Alectoris rufa) de-novo transcriptome assembly and identification of gene-related markers[J],2017,11:132-134.
APA	Sevane, Natalia,Canon, Javier,Eusebi, Paulina G.,Gil, Ignacio,&Dunner, Susana.(2017).Red-legged partridge (Alectoris rufa) de-novo transcriptome assembly and identification of gene-related markers.GENOMICS DATA,11,132-134.
MLA	Sevane, Natalia,et al."Red-legged partridge (Alectoris rufa) de-novo transcriptome assembly and identification of gene-related markers".GENOMICS DATA 11(2017):132-134.