干旱区生态环境知识资源中心(Arid): Simultaneous determination of seven aconitum alkaloids in bioloaical samples by liquid chromatography-linear ion trap multistage mass spectrometry coupled with on-line solid phase extraction

Arid

DOI	10.3724/SP.J.1123.2015.10044
	Simultaneous determination of seven aconitum alkaloids in bioloaical samples by liquid chromatography-linear ion trap multistage mass spectrometry coupled with on-line solid phase extraction
	Zhu Dingji; Lu Minping; Huang Kejian; Zhou Zhe; Lin Cuiwu; Yang Ning; Liu Xiaofeng; Qiao Wentao; Li Lu; Huang Xiaoqing
通讯作者	Huang, KJ
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2016
卷号	34 期号:3 页码:249-257
英文摘要	An analytical method has been developed for the simultaneous determination of seven aconitum alkaloids in biological samples by liquid chromatography-linear ion trap multistage mass spectrometry (LC-LIT/MSn) coupled with on-line solid phase extraction (on-line SPE). Mass fragmentation regularities of the seven aconitum alkaloids are discussed and summarized according to the analysis of their MSn fragments. The samples were treated with acetonitrile for protein precipitation, followed by dilution and centrifugation. The resulting solution was injected into the LC system directly and processed by Waters Oasis HLB on-line SPE column for enrichment and purification. Chromatographic separation was performed on an Accucore C18 column using mobile phases of 0.01 mol/L ammonium acetate aqueous solution and methanol (both containing 0.1% (v/v) formic acid) for gradient elution. The analytes were detected in consecutive reaction monitoring (CRM) mode through positive electrospray ionization (EST+). The calibration curves of the analytes within the investigated mass concentration ranges were fitted by second-order equations weighted 1/x, with r(2) ranging from 0. 999 1 to 0.999 9. The method detection limits were 0. 02-0. 60 ng/mL for whole blood and urine, 0. 02-0. 40 ng/g for liver tissue. The recoveries at three spiked levels were within 91. 1%-104. 7% for all the analytes, with 0. 2%-10. 7% of intra-day RSDs and 1. 0%-13. 7% of inter-day RSDs (n = 6). The established method is simple, accurate, and sensitive for rapid determination of the seven aconitum alkaloids in biological samples.
英文关键词	on-line solid phase extraction (on-line SPE) liquid chromatography-linear ion trap multistage mass spectrometry (LC-LIT/MSn) consecutive reaction monitoring (CRM) aconitum alkaloids biological samples
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000408916200005
WOS关键词	HUMAN URINE
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/331887
作者单位	[Zhu Dingji] Guangxi Zhuang Autonomous Reg Acad Prevent & Trea, Nanning 530021, Peoples R China; [Lu Minping; Lin Cuiwu] Guangxi Univ, Sch Chem & Chem Engn, Nanning 530004, Peoples R China; [Huang Kejian; Yang Ning; Liu Xiaofeng; Qiao Wentao; Li Lu; Huang Xiaoqing] Guangxi Zhuang Autonomous Reg Publ Secur Dept, Evidence Identificat Ctr, Nanning 530012, Peoples R China; [Zhou Zhe] Thermo Fisher Sci China Co Ltd, Shanghai 201206, Peoples R China
推荐引用方式 GB/T 7714	Zhu Dingji,Lu Minping,Huang Kejian,et al. Simultaneous determination of seven aconitum alkaloids in bioloaical samples by liquid chromatography-linear ion trap multistage mass spectrometry coupled with on-line solid phase extraction[J],2016,34(3):249-257.
APA	Zhu Dingji.,Lu Minping.,Huang Kejian.,Zhou Zhe.,Lin Cuiwu.,...&Huang Xiaoqing.(2016).Simultaneous determination of seven aconitum alkaloids in bioloaical samples by liquid chromatography-linear ion trap multistage mass spectrometry coupled with on-line solid phase extraction.CHINESE JOURNAL OF CHROMATOGRAPHY,34(3),249-257.
MLA	Zhu Dingji,et al."Simultaneous determination of seven aconitum alkaloids in bioloaical samples by liquid chromatography-linear ion trap multistage mass spectrometry coupled with on-line solid phase extraction".CHINESE JOURNAL OF CHROMATOGRAPHY 34.3(2016):249-257.