干旱区生态环境知识资源中心(Arid): Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity

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DOI	10.4103/2231-4040.177201
	Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity
	Ibeyaima, A.; Rana, Jyoti; Dwivedi, Anuj; Gupta, Sanjay; Sharma, Sanjeev K.; Saini, Narendra; Sarethy, Indira P.
通讯作者	Sarethy, IP
来源期刊	JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH
ISSN	2231-4040
EISSN	0976-2094
出版年	2016
卷号	7 期号:2 页码:32-36
英文摘要	During a screening program for antimicrobial compounds from underexplored habitats, a Gram-positive bacterium TD-032, was isolated from arid soil, Thar Desert (India), and analyzed for its morphological, physicochemical, and antimicrobial properties. The 16S ribosomal DNA (rDNA) sequence of the isolate was further studied for the novelty of -hyper variable region. TD-032 was grown in large-scale culture, and aqueous and organic solvent extracts analyzed for antimicrobial activity. Culture characteristics showed a lack of diffusible and melanoid pigments. The morphological features were pale yellow aerial mycelium colony color with brownish yellow substrate mycelium and leathery texture. The isolate could grow at 1% concentration of sodium chloride, temperature of 40?C, and a wide range of pH (7.0-12.0). An evaluation for extracellular enzymatic activities showed secretion of gelatinase(s), cellulase(s), and lipase(s). The -hyper variable region of 16S rDNA sequence of TD-032 showed 98.33% relatedness to Yuhushiella deserti, indicating a potential new species. Aqueous and ethyl acetate extracts showed antimicrobial activity against Gram-positive and Gram-negative bacteria inclusive clinical isolates. Inhibition of both test bacteria suggests that TD-032 produces a broad spectrum of antimicrobial substances.
英文关键词	Actinomycetes antimicrobial activity hyper-variable regions 16S ribosomal DNA Thar Desert
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000374230400002
WOS关键词	SP NOV. ; SOIL ; CHAXAMYCINS ; DIVERSITY
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/331513
作者单位	[Ibeyaima, A.; Rana, Jyoti; Dwivedi, Anuj; Gupta, Sanjay; Sharma, Sanjeev K.; Sarethy, Indira P.] Jaypee Inst Informat Technol, Dept Biotechnol, A-10,Sect 62, Noida 201307, Uttar Pradesh, India; [Saini, Narendra] Pushpanjali Crosslay Hosp, Dept Microbiol, Ghaziabad, Uttar Pradesh, India
推荐引用方式 GB/T 7714	Ibeyaima, A.,Rana, Jyoti,Dwivedi, Anuj,et al. Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity[J],2016,7(2):32-36.
APA	Ibeyaima, A..,Rana, Jyoti.,Dwivedi, Anuj.,Gupta, Sanjay.,Sharma, Sanjeev K..,...&Sarethy, Indira P..(2016).Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity.JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH,7(2),32-36.
MLA	Ibeyaima, A.,et al."Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity".JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH 7.2(2016):32-36.