干旱区生态环境知识资源中心(Arid): Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers

Arid

DOI	10.1007/s40003-015-0165-7
	Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers
	Sharma, Vikas; Sharma, Tilak Raj; Rana, Jai Chand; Chahota, Rakesh Kumar
通讯作者	Chahota, RK
来源期刊	AGRICULTURAL RESEARCH
ISSN	2249-720X
EISSN	2249-7218
出版年	2015
卷号	4 期号:3 页码:221-230
英文摘要	Macrotyloma uniflorum (horsegram) is an underutilized warm season pulse crop used as food and fodder in many semi arid regions of the world. In the present study, genetic structure and diversity of M. uniflorum was analysed using RAPD and ISSR markers. Two other species of the genus Macrotyloma namely M. axillare and M. sar-gharwalensis were also included to assess genetic inter-relationships. In total, 25 polymorphic primers amplified 156 fragments ranging in size from 300 to 3000 bp. Primer wise fragments ranged from 2 (OPR-20) to 13 (OPB-5, OPB-12 and OPS-14) with an average of 6.24 fragments per primer. Highest PIC value of 0.499 was recorded for primer OPR-20 and lowest (0.013) for primer OPR-2 with an average of 0.344. STRUCTURE analysis clustered accessions on the basis of their geographic origin and showed the presence of two distinct gene pools. Dendrogram based on Jaccards similarity coefficient also grouped the accessions into two groups and revealed that M. sar-gharwalensis is more distantly related to the cultivated than M. axillare. PCA analysis also confirmed clustering results shown by STRUCTURE. Partitioning of genetic variation using AMOVA revealed 63 % within population variation with only 37 % genetic variation among populations. Few horsegram accessions showed high levels of genetic diversity which can be exploited in crop breeding programmes for its genetic improvement.
英文关键词	Horsegram Genetic diversity Genetic structure RAPD ISSR
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000366136200001
WOS关键词	MULTILOCUS GENOTYPE DATA ; POLYMORPHISM MARKERS ; AFLP MARKERS ; LAM. VERDC. ; SSR MARKERS ; L. ; TRANSFERABILITY ; INFERENCE ; LEGUMES
WOS类目	Agronomy
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/331177
作者单位	[Sharma, Vikas; Sharma, Tilak Raj; Chahota, Rakesh Kumar] CSKHP Agr Univ, Dept Agr Biotechnol, Palampur 176062, Himachal Prades, India; [Rana, Jai Chand] Natl Bur Plant Genet Resources, Reg Stn, Shimla, HP, India
推荐引用方式 GB/T 7714	Sharma, Vikas,Sharma, Tilak Raj,Rana, Jai Chand,et al. Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers[J],2015,4(3):221-230.
APA	Sharma, Vikas,Sharma, Tilak Raj,Rana, Jai Chand,&Chahota, Rakesh Kumar.(2015).Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers.AGRICULTURAL RESEARCH,4(3),221-230.
MLA	Sharma, Vikas,et al."Analysis of Genetic Diversity and Population Structure in Horsegram (Macrotyloma uniflorum) Using RAPD and ISSR Markers".AGRICULTURAL RESEARCH 4.3(2015):221-230.