干旱区生态环境知识资源中心(Arid): METHOD DEVELOPMENT FOR THE ANALYSIS OF PHARMACEUTICALS WITH ACETHYLCHOLINESTERASE ACTIVITY IN WATER USING HPLC-DAD AND SOLID PHASE EXTRACTION

Arid

DOI	10.22146/ijc.21263
	METHOD DEVELOPMENT FOR THE ANALYSIS OF PHARMACEUTICALS WITH ACETHYLCHOLINESTERASE ACTIVITY IN WATER USING HPLC-DAD AND SOLID PHASE EXTRACTION
	Wardhana, Samuel Budi; Kolb, Marit; Bahadir, Muefit; Purwono, Bambang; Wahyuningsih, Tutik Dwi
通讯作者	Wardhana, SB
来源期刊	INDONESIAN JOURNAL OF CHEMISTRY
ISSN	1411-9420
出版年	2014
卷号	14 期号:1 页码:22-31
英文摘要	An SPE followed by HPLC-DAD method with ion pair chromatography technique to analyze pharmaceuticals with acethylcholinesterase activity including pyridostigmine (PYR), galathamine (GAL), neostigmine (NEO), eserine (ESE), and donepezil (DON) in water samples was developed. Acetylcholinesterase (AChE) inhibitors have been used to treat less severe dementias such as Alzheimer's disease. Chromatographic separation was achieved using reversed-phase SymmetryShield column using gradient system with mobile phase consisting of H2O/ACN (99: 1, v/v) as mobile phase A with 10 mM sodium 1-hexanesulfonate and 0.1% acetic acid (HAc). The HPLC/DAD method was linear between concentrations of 5 to 100 ng/mu L. The IDL and IQL ranged from 0.50 to 1.25 ng/mu L and 1.5 to 3.0 ng/mu L, respectively. SPE was used to extract and clean up the target substances in spiked pure water, tap water, and wastewater samples. The application of extraction method of 5 target substances in wastewater sample was divided into 2 parts: Oasis WCX (6 mL, 500 mg) for PYR and Oasis HLB (6 mL, 200 mg) for GAL, NEO, ESE and DON. The developed SPE and HPLC/DAD method is applicable for quantification of the 5 target substances in water samples in a concentration range >50 mu g/L and assumable lower for DON (> 25 mu g/L).
英文关键词	acetylcholinesterase solid phase extraction ion pair chromatography
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000420622500004
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330890
作者单位	[Wardhana, Samuel Budi] Semarang State Univ, Dept Chem, Kampus Sakaran, Central Java 50229, Indonesia; [Kolb, Marit] Tech Univ Carolo Wilhelmina Braunschweig, Inst Environm & Sustainable Chem, D-38106 Braunschweig, Germany; [Purwono, Bambang; Wahyuningsih, Tutik Dwi] Univ Gadjah Mada, Fac Math & Nat Sci, Dept Chem, Yogyakarta 55281, Indonesia
推荐引用方式 GB/T 7714	Wardhana, Samuel Budi,Kolb, Marit,Bahadir, Muefit,et al. METHOD DEVELOPMENT FOR THE ANALYSIS OF PHARMACEUTICALS WITH ACETHYLCHOLINESTERASE ACTIVITY IN WATER USING HPLC-DAD AND SOLID PHASE EXTRACTION[J],2014,14(1):22-31.
APA	Wardhana, Samuel Budi,Kolb, Marit,Bahadir, Muefit,Purwono, Bambang,&Wahyuningsih, Tutik Dwi.(2014).METHOD DEVELOPMENT FOR THE ANALYSIS OF PHARMACEUTICALS WITH ACETHYLCHOLINESTERASE ACTIVITY IN WATER USING HPLC-DAD AND SOLID PHASE EXTRACTION.INDONESIAN JOURNAL OF CHEMISTRY,14(1),22-31.
MLA	Wardhana, Samuel Budi,et al."METHOD DEVELOPMENT FOR THE ANALYSIS OF PHARMACEUTICALS WITH ACETHYLCHOLINESTERASE ACTIVITY IN WATER USING HPLC-DAD AND SOLID PHASE EXTRACTION".INDONESIAN JOURNAL OF CHEMISTRY 14.1(2014):22-31.