干旱区生态环境知识资源中心(Arid): Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halophyte Aeluropus lagopoides: Identification and Characterization

Arid

DOI	10.1080/15427528.2013.766294
	Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halophyte Aeluropus lagopoides: Identification and Characterization
	Modarresi, Mostafa; Nematzadeh, Ghorban Ali; Zarein, Mohammad
通讯作者	Modarresi, M
来源期刊	JOURNAL OF CROP IMPROVEMENT
ISSN	1542-7528
EISSN	1542-7536
出版年	2013
卷号	27 期号:3 页码:281-290
英文摘要	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which plays an important role in central carbon metabolism, was studied in Aeluropus lagopoides, which is an important perennial grass found in drought-stricken areas and deserts from Africa to South Asia. Aeluropus lagopoides is an ideal candidate plant system to identify novel or enhanced stress tolerance mechanisms. The GAPDH has stable expression under different conditions and it is a typical housekeeping gene in prokaryotes and eukaryotes. So far, there are no reports about identification of housekeeping genes for gene expression analysis in Aeluropus lagopoides. Thus in the present study, a GAPDH cDNA was cloned and sequenced from this plant. Our results revealed that the open reading frame (ORF) (1014 bp) encoded a polypeptide of 337 amino-acid residues. The deduced amino acid sequence indicated a protein with a molecular weight of 364.95 kDa and estimated isoelectric point value of 7.06. Multiple alignments of putative amino acid sequences indicated high similarity between Aeluropus lagopoides GAPDH and analogous protein derived from other plants, particularly monocots. Nucleotide blast and phylogenetic analysis revealed that this plant had maximum identity with Cleistogenes songorica. Results of this experiment can help us do transcriptional analysis by qRT-PCR to identify stress-tolerance mechanisms.
英文关键词	cDNA gene cloning housekeeping gene
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000452721400003
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330621
作者单位	[Modarresi, Mostafa] Tarbiat Modares Univ, Dept Plant Breeding, Fac Agr, Tehran, Iran; [Modarresi, Mostafa; Nematzadeh, Ghorban Ali] Sari Agr Sci & Nat Resources Univ, Genet & Agr Biotechnol Inst Tabarestan, Sari, Iran; [Zarein, Mohammad] Tarbiat Modares Univ, Fac Agr, Dept Agr Machinery Engn, Tehran, Iran
推荐引用方式 GB/T 7714	Modarresi, Mostafa,Nematzadeh, Ghorban Ali,Zarein, Mohammad. Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halophyte Aeluropus lagopoides: Identification and Characterization[J],2013,27(3):281-290.
APA	Modarresi, Mostafa,Nematzadeh, Ghorban Ali,&Zarein, Mohammad.(2013).Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halophyte Aeluropus lagopoides: Identification and Characterization.JOURNAL OF CROP IMPROVEMENT,27(3),281-290.
MLA	Modarresi, Mostafa,et al."Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halophyte Aeluropus lagopoides: Identification and Characterization".JOURNAL OF CROP IMPROVEMENT 27.3(2013):281-290.