干旱区生态环境知识资源中心(Arid): Construction of a Recombinant Allergen-Producing Probiotic Bacterial Strain: Introduction of a New Line for a Live Oral Vaccine Against Chenopodium album Pollen Allergy

Arid

	Construction of a Recombinant Allergen-Producing Probiotic Bacterial Strain: Introduction of a New Line for a Live Oral Vaccine Against Chenopodium album Pollen Allergy
	Nasiraie, Leila Roozbeh; Tabatabaie, Farideh; Sankian, Mojtaba; Shahidi, Fakhri; Varasteh, Abdolreza
通讯作者	Varasteh, A
来源期刊	REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	2322-3480
出版年	2013
卷号	2 期号:1 页码:16-27
英文摘要	Background: During the last two decades, significant advances have been made in the fields of lactococcal genetics and protein expression. Lactococcus lactis (L. lactis) is an effective vector for protein expression and can be used as an antigen delivery system. Hence, L. lactis is an ideal candidate for mucosal immunotherapy. Profilin (Che a 2), the major allergen in Chenopodium album, is one of the most important causes of allergic diseases in desert and semi-desert areas, especially in Iran, Saudi Arabia, and Kuwait that was cloned and expressed in L. lactis for the first time. Methods: To construct L. lactis that expressed Che a 2, a DNA sequence was cloned and used to transform bacteria. Expression of Che a 2 was analyzed via monitoring of related RNA and protein. Hydrophobicity, adherence to HT-29 cells, antibiotic resistance, resistance to gastrointestinal contents, pH, and bile salt in recombinant and native L. lactis were evaluated. Results: Immunoblot analyses demonstrated that recombinant Che a 2 is expressed as a 32 kDa dimeric protein immunological studies showed it can bind human IgE. Both native and recombinant bacteria were sensitive to low pH and simulated gastric conditions. Bacterial survival was reduced 80-100% after 2 h of exposure to pH 1.5-2. Both native and recombinant bacteria were able to grow in 0.3 and 2% bile salts. After incubation of recombinant L. lactis in simulated gastric and intestinal juices for one and two hours, respectively, cell survival was reduced by 100%. Adhesion capability in both strains was minimal and there were no significant differences in any of our tests between native and recombinant bacteria. Conclusion: Successfully recombinant L. lactis with capability of expression Che a 2 was produced and revealed it is sensitive to gastrointestinal contents.
英文关键词	Chenopodium pollen allergen Oral vaccines Probiotic bacteria Recombinant L. lactis
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000446893700002
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330605
作者单位	[Nasiraie, Leila Roozbeh; Tabatabaie, Farideh; Shahidi, Fakhri] Ferdowsi Univ Mashhad, Dept Food Sci & Technol, Mashhad, Iran; [Sankian, Mojtaba] Mashhad Univ Med Sci, Sch Med, Immunol Res Ctr, Immunobiochem Lab, Mashhad, Iran; [Varasteh, Abdolreza] Mashhad Univ Med Sci, Sch Med, Allergy Res Ctr, Immunobiochem Lab, Mashhad, Iran
推荐引用方式 GB/T 7714	Nasiraie, Leila Roozbeh,Tabatabaie, Farideh,Sankian, Mojtaba,et al. Construction of a Recombinant Allergen-Producing Probiotic Bacterial Strain: Introduction of a New Line for a Live Oral Vaccine Against Chenopodium album Pollen Allergy[J],2013,2(1):16-27.
APA	Nasiraie, Leila Roozbeh,Tabatabaie, Farideh,Sankian, Mojtaba,Shahidi, Fakhri,&Varasteh, Abdolreza.(2013).Construction of a Recombinant Allergen-Producing Probiotic Bacterial Strain: Introduction of a New Line for a Live Oral Vaccine Against Chenopodium album Pollen Allergy.REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY,2(1),16-27.
MLA	Nasiraie, Leila Roozbeh,et al."Construction of a Recombinant Allergen-Producing Probiotic Bacterial Strain: Introduction of a New Line for a Live Oral Vaccine Against Chenopodium album Pollen Allergy".REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 2.1(2013):16-27.