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DOI	10.1016/j.jpha.2012.09.004
	A rapid and sensitive liquid chromatography tandem mass spectrometric assay for duloxetine in human plasma: Its pharmacokinetic application
	Gajula, Ramakrishna; Maddela, Rambabu; Ravi, Vasu Babu; Inamadugu, Jaswanth Kumar; Pilli, Nageswara Rao
通讯作者	Inamadugu, JK
来源期刊	JOURNAL OF PHARMACEUTICAL ANALYSIS
ISSN	2095-1779
EISSN	2214-0883
出版年	2013
卷号	3 期号:1 页码:36-44
英文摘要	This paper describes a simple, rapid and sensitive liquid chromatography tandem mass spectrometry assay for the determination of duloxetine in human plasma. A duloxetine stable labeled isotope (duloxetine d(5)) was used as an internal standard. Analyte and the internal standard were extracted from 100 mu L of human plasma via solid phase extraction technique using Oasis HLB cartridges. The chromatographic separation was achieved on a C-18 column by using a mixture of acetonitrile-5 mM ammonium acetate buffer (83:17, v/v) as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve obtained was linear (r(2) >= 0.99) over the concentration range of 0.05-101 ng/mL. Multiple-reaction monitoring mode (MRM) was used for quantification of ion transitions at m/z 298.3/154.1 and 303.3/159.1 for the drug and the internal standard, respectively. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. (C) 2013 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. All rights reserved.
英文关键词	Duloxetine in human plasma Solid-phase extraction (SPE) Liquid chromatography tandem mass spectrometry Method validation Pharmacokinetic studies
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000218927400005
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330424
作者单位	[Gajula, Ramakrishna; Maddela, Rambabu; Ravi, Vasu Babu; Inamadugu, Jaswanth Kumar; Pilli, Nageswara Rao] Wellquest Clin Res Labs, Hyderabad 500013, Andhra Pradesh, India
推荐引用方式 GB/T 7714	Gajula, Ramakrishna,Maddela, Rambabu,Ravi, Vasu Babu,et al. A rapid and sensitive liquid chromatography tandem mass spectrometric assay for duloxetine in human plasma: Its pharmacokinetic application[J],2013,3(1):36-44.
APA	Gajula, Ramakrishna,Maddela, Rambabu,Ravi, Vasu Babu,Inamadugu, Jaswanth Kumar,&Pilli, Nageswara Rao.(2013).A rapid and sensitive liquid chromatography tandem mass spectrometric assay for duloxetine in human plasma: Its pharmacokinetic application.JOURNAL OF PHARMACEUTICAL ANALYSIS,3(1),36-44.
MLA	Gajula, Ramakrishna,et al."A rapid and sensitive liquid chromatography tandem mass spectrometric assay for duloxetine in human plasma: Its pharmacokinetic application".JOURNAL OF PHARMACEUTICAL ANALYSIS 3.1(2013):36-44.