Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.6026/97320630081211 |
Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs | |
Kumari, Archana; Kumar, Ashutosh; Wany, Aakanksha; Prajapati, Gopal Kumar; Pandey, Dev Mani | |
通讯作者 | Pandey, DM |
来源期刊 | BIOINFORMATION
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ISSN | 0973-8894 |
EISSN | 0973-2063 |
出版年 | 2012 |
卷号 | 8期号:24页码:1211-1219 |
英文摘要 | Peanut (Arachis hypogaea L.) ranks fifth among the world oil crops and is widely grown in India and neighbouring countries. Due to its large and unknown genome size, studies on genomics and genetic modification of peanut are still scanty as compared to other model crops like Arabidopsis, rice, cotton and soybean. Because of its favourable cultivation in semi-arid regions, study on abiotic stress responsive genes and its regulation in peanut is very much important. Therefore, we aim to identify and annotate the abiotic stress responsive candidate genes in peanut ESTs. Expression data of drought stress responsive corresponding genes and EST sequences were screened from dot blot experiments shown as heat maps and supplementary tables, respectively as reported by Govind et al. (2009). Some of the screened genes having no information about their ESTs in above mentioned supplementary tables were retrieved from NCBI. A phylogenetic analysis was performed to find a group of utmost similar ESTs for each selected gene. Individual EST of the said group were further searched in peanut ESTs (1,78,490 whole EST sequences) using stand alone BLAST. For the prediction as well as annotation of abiotic stress responsive selected genes, various tools (like Vec-Screen, Repeat Masker, EST-Trimmer, DNA Baser, WISE2 and I-TASSER) were used. Here we report the predicted result of Contigs, domain as well as 3D structure for HSP 17.3KDa protein, DnaJ protein and Type 2 Metallothionein protein. |
英文关键词 | Arachis hypogaea EST Gene annotation Stress Contigs |
类型 | Article |
语种 | 英语 |
开放获取类型 | Green Published, Other Gold |
收录类别 | ESCI |
WOS记录号 | WOS:000449435700006 |
WOS关键词 | EXPRESSED SEQUENCE TAGS ; HEAT-SHOCK PROTEINS ; GENERATION ; DISCOVERY ; GENOMICS ; PLANTS |
WOS类目 | Mathematical & Computational Biology |
WOS研究方向 | Mathematical & Computational Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/330236 |
作者单位 | [Kumari, Archana; Kumar, Ashutosh; Wany, Aakanksha; Prajapati, Gopal Kumar; Pandey, Dev Mani] Birla Inst Technol, Dept Biotechnol, Ranchi 835215, Jharkhand, India |
推荐引用方式 GB/T 7714 | Kumari, Archana,Kumar, Ashutosh,Wany, Aakanksha,et al. Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs[J],2012,8(24):1211-1219. |
APA | Kumari, Archana,Kumar, Ashutosh,Wany, Aakanksha,Prajapati, Gopal Kumar,&Pandey, Dev Mani.(2012).Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs.BIOINFORMATION,8(24),1211-1219. |
MLA | Kumari, Archana,et al."Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs".BIOINFORMATION 8.24(2012):1211-1219. |
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