Arid
DOI10.6026/97320630081211
Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs
Kumari, Archana; Kumar, Ashutosh; Wany, Aakanksha; Prajapati, Gopal Kumar; Pandey, Dev Mani
通讯作者Pandey, DM
来源期刊BIOINFORMATION
ISSN0973-8894
EISSN0973-2063
出版年2012
卷号8期号:24页码:1211-1219
英文摘要Peanut (Arachis hypogaea L.) ranks fifth among the world oil crops and is widely grown in India and neighbouring countries. Due to its large and unknown genome size, studies on genomics and genetic modification of peanut are still scanty as compared to other model crops like Arabidopsis, rice, cotton and soybean. Because of its favourable cultivation in semi-arid regions, study on abiotic stress responsive genes and its regulation in peanut is very much important. Therefore, we aim to identify and annotate the abiotic stress responsive candidate genes in peanut ESTs. Expression data of drought stress responsive corresponding genes and EST sequences were screened from dot blot experiments shown as heat maps and supplementary tables, respectively as reported by Govind et al. (2009). Some of the screened genes having no information about their ESTs in above mentioned supplementary tables were retrieved from NCBI. A phylogenetic analysis was performed to find a group of utmost similar ESTs for each selected gene. Individual EST of the said group were further searched in peanut ESTs (1,78,490 whole EST sequences) using stand alone BLAST. For the prediction as well as annotation of abiotic stress responsive selected genes, various tools (like Vec-Screen, Repeat Masker, EST-Trimmer, DNA Baser, WISE2 and I-TASSER) were used. Here we report the predicted result of Contigs, domain as well as 3D structure for HSP 17.3KDa protein, DnaJ protein and Type 2 Metallothionein protein.
英文关键词Arachis hypogaea EST Gene annotation Stress Contigs
类型Article
语种英语
开放获取类型Green Published, Other Gold
收录类别ESCI
WOS记录号WOS:000449435700006
WOS关键词EXPRESSED SEQUENCE TAGS ; HEAT-SHOCK PROTEINS ; GENERATION ; DISCOVERY ; GENOMICS ; PLANTS
WOS类目Mathematical & Computational Biology
WOS研究方向Mathematical & Computational Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/330236
作者单位[Kumari, Archana; Kumar, Ashutosh; Wany, Aakanksha; Prajapati, Gopal Kumar; Pandey, Dev Mani] Birla Inst Technol, Dept Biotechnol, Ranchi 835215, Jharkhand, India
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Kumari, Archana,Kumar, Ashutosh,Wany, Aakanksha,et al. Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs[J],2012,8(24):1211-1219.
APA Kumari, Archana,Kumar, Ashutosh,Wany, Aakanksha,Prajapati, Gopal Kumar,&Pandey, Dev Mani.(2012).Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs.BIOINFORMATION,8(24),1211-1219.
MLA Kumari, Archana,et al."Identification and annotation of abiotic stress responsive candidate genes in peanut ESTs".BIOINFORMATION 8.24(2012):1211-1219.
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