干旱区生态环境知识资源中心(Arid): Sequence similarity based identification of abiotic stress responsive genes in chickpea

Arid

	Sequence similarity based identification of abiotic stress responsive genes in chickpea
	Roorkiwal, Manish; Sharma, Prakash Chand
通讯作者	Sharma, PC
来源期刊	BIOINFORMATION
ISSN	0973-8894
EISSN	0973-2063
出版年	2012
卷号	8 期号:2 页码:92-97
英文摘要	Chickpea (Cicer arietinum L.) is an important food legume crop, particularly for the arid regions including Indian subcontinent. Considering the detrimental effect of drought, temperature and salt stress on crop yield, efforts have been initiated in the direction of developing improved varieties and designing alternate strategies to sustain chickpea production in adverse environmental conditions. Identification of genes that confer abiotic stress tolerance in plants remains a challenge in contemporary plant breeding. The present study focused on the identification of abiotic stress responsive genes in chickpea based on sequence similarity approach exploiting known abiotic stress responsive genes from model crops or other plant species. Ten abiotic stress responsive genes identified in other plants were partially amplified from eight chickpea genotypes and their presence in chickpea was confirmed after sequencing the PCR products. These genes have been functionally validated and reported to play significant role in stress response in model plants like Arabidopsis, rice and other legume crops. Chickpea EST sequences available at NCBI EST database were used for the identification of abiotic stress responsive genes. A total of 8,536 unique coding long sequences were used for identification of chickpea homologues of these abiotic stress responsive genes by sequence similarity search (BLASTN and BLASTX). These genes can be further explored towards achieving the goal of developing superior chickpea varieties providing improved yields under stress conditions using modern molecular breeding approaches.
英文关键词	Chickpea Abiotic stress Candidate genes Sequence similarity
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000449407100006
WOS类目	Mathematical & Computational Biology
WOS研究方向	Mathematical & Computational Biology
来源机构	International Crops Research Institute for the Semi-Arid Tropics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330235
作者单位	[Roorkiwal, Manish; Sharma, Prakash Chand] Guru Gobind Singh Indraprastha Univ, Univ Sch Biotechnol, Dwarka Sect 16C, New Delhi 110075, India; [Roorkiwal, Manish] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India
推荐引用方式 GB/T 7714	Roorkiwal, Manish,Sharma, Prakash Chand. Sequence similarity based identification of abiotic stress responsive genes in chickpea[J]. International Crops Research Institute for the Semi-Arid Tropics,2012,8(2):92-97.
APA	Roorkiwal, Manish,&Sharma, Prakash Chand.(2012).Sequence similarity based identification of abiotic stress responsive genes in chickpea.BIOINFORMATION,8(2),92-97.
MLA	Roorkiwal, Manish,et al."Sequence similarity based identification of abiotic stress responsive genes in chickpea".BIOINFORMATION 8.2(2012):92-97.