干旱区生态环境知识资源中心(Arid): USE OF THE ISSR MARKERS FOR THE STUDY OF GENETIC POLYMORPHISM OF THE PISTACHIO FRUIT PISTACIA VERA L. IN ALGERIA

Arid

	USE OF THE ISSR MARKERS FOR THE STUDY OF GENETIC POLYMORPHISM OF THE PISTACHIO FRUIT PISTACIA VERA L. IN ALGERIA
	Kebour, Djamila; Boutekrabt, Ammar; Mefti, Med; Abdul Hussain, Alia Simona
通讯作者	Kebour, D
来源期刊	UNIVERSITY POLITEHNICA OF BUCHAREST SCIENTIFIC BULLETIN SERIES B-CHEMISTRY AND MATERIALS SCIENCE
ISSN	1454-2331
出版年	2012
卷号	74 期号:2 页码:191-200
英文摘要	Pistacia vera L. is a widely presented plant in Algerian semi-arid regions. It is potentially usable to restore degraded ecosystems. Genetic relationships among the cultivars was assessed by using six inter simple repeat (ISSR) primers. During the ISSR screening in this study, good amplification products were obtained from primers based on GA (guanine-adenine), CA and GAA repeats. But primers based on cytosine-thyrosine (CT) and CAA repeats produced few large separate bands, so these primers were not selected for the final analysis (eliminated for the final analysis). The present study showed that ISSR-PCR analysis is quick, reliable and produces sufficient polymorphisms for large-scale DNA fingerprinting purposes. The total of 111 bands of which 60 were polymorphic, (with 54.04%) was amplified by the six primers, an average of seven bands per primer. The total number of amplified fragments was between five to ten and the number of polymorphic fragments ranged from four to seven. The range of genetic similarity was from 0/84 to 1. The present study showed that there is low genetic diversity among tested cultivars and the ISSR-PCR analysis produces sufficient polymorphisms for large-scale DNA fingerprinting. This study reports the first application of the ISSR technique in characterization of Algerian pistachio cultivars originary from Syria.
英文关键词	Pistachio pistacia vera L. Genetic relationships DNA extraction ISSR Clustering
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000440233600015
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330208
作者单位	[Kebour, Djamila; Boutekrabt, Ammar] Univ BLIDA, Lab Biotechnol, Blida, Algeria; [Mefti, Med] Univ BLIDA, Lab Biotechnol, Blida, Algeria; [Abdul Hussain, Alia Simona] Univ BLIDA, Blida, Algeria
推荐引用方式 GB/T 7714	Kebour, Djamila,Boutekrabt, Ammar,Mefti, Med,et al. USE OF THE ISSR MARKERS FOR THE STUDY OF GENETIC POLYMORPHISM OF THE PISTACHIO FRUIT PISTACIA VERA L. IN ALGERIA[J],2012,74(2):191-200.
APA	Kebour, Djamila,Boutekrabt, Ammar,Mefti, Med,&Abdul Hussain, Alia Simona.(2012).USE OF THE ISSR MARKERS FOR THE STUDY OF GENETIC POLYMORPHISM OF THE PISTACHIO FRUIT PISTACIA VERA L. IN ALGERIA.UNIVERSITY POLITEHNICA OF BUCHAREST SCIENTIFIC BULLETIN SERIES B-CHEMISTRY AND MATERIALS SCIENCE,74(2),191-200.
MLA	Kebour, Djamila,et al."USE OF THE ISSR MARKERS FOR THE STUDY OF GENETIC POLYMORPHISM OF THE PISTACHIO FRUIT PISTACIA VERA L. IN ALGERIA".UNIVERSITY POLITEHNICA OF BUCHAREST SCIENTIFIC BULLETIN SERIES B-CHEMISTRY AND MATERIALS SCIENCE 74.2(2012):191-200.