干旱区生态环境知识资源中心(Arid): Fast determination of adenosine and cordycepin in Cordyceps and its deserted solid medium

Arid

DOI	10.3724/SP.J.1123.2012.02037
	Fast determination of adenosine and cordycepin in Cordyceps and its deserted solid medium
	Li Chen; Yan Aiguo; Cai Chunyan; Liu Zhiping
通讯作者	Li, C
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2012
卷号	30 期号:7 页码:711-715
英文摘要	A fast analytical method for adenosine and cordycepin in Cordyceps and its deserted solid medium was developed by using a normal-phase cyan-group chromatographic column. The sample was extracted for 1. 5 min using a microwave-assisted extraction system. The extraction was repeated twice. The analysis of adenosine and cordycepin was performed on an Eclipse XDB-CN column. The mobile phase was composed of methanol and water with a ratio of 7: 93 v/v for isocratic elution. The detection wavelength was 260 nm. The composition and pH value of the mobile phase were investigated. The results showed that adenosine and cordycepin could be completely separated without matrix interference in 4. 5 min. The linearity of the method was good with a linear correlation coefficient r(2) of 0. 999 8 for adenosine and 0.999 5 for cordycepin. The limits of quantification LOQs S/N = 10 of adenosine and cordycepin were 0. 21 and 0. 083 mg/L respectively. The relative standard deviations RSDs of peak areas of six replicate injections were less than 2% both for intro-day and inter-day analysis. The average recoveries of adenosine and cordycepin ranged from 93.8% to 102. 9% with the RSD not more than 3. 62% n = 5 . The developed method is simple fast accurate and low-cost and it can be used in the fast detection of adenosine and cordycepin in Cordyceps carpohole the deserted solid medium and its praeparatum.
英文关键词	normal-phase chromatographic column adenosine cordycepin Cordyceps deserted solid medium
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435252300011
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330181
作者单位	[Li Chen] Guangdong Univ Technol, Fac Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China; [Li Chen; Liu Zhiping] Wu Yi Univ, Instrumental Anal & Res Ctr, Jiangmen 529020, Peoples R China; [Yan Aiguo] Jiangmen Qual Supervis & Inspect Ctr Agr Prod, Jiangmen 529020, Peoples R China; [Cai Chunyan] Jiangmen Monitoring Ctr Agr Environm, Jiangmen 529020, Peoples R China
推荐引用方式 GB/T 7714	Li Chen,Yan Aiguo,Cai Chunyan,et al. Fast determination of adenosine and cordycepin in Cordyceps and its deserted solid medium[J],2012,30(7):711-715.
APA	Li Chen,Yan Aiguo,Cai Chunyan,&Liu Zhiping.(2012).Fast determination of adenosine and cordycepin in Cordyceps and its deserted solid medium.CHINESE JOURNAL OF CHROMATOGRAPHY,30(7),711-715.
MLA	Li Chen,et al."Fast determination of adenosine and cordycepin in Cordyceps and its deserted solid medium".CHINESE JOURNAL OF CHROMATOGRAPHY 30.7(2012):711-715.