干旱区生态环境知识资源中心(Arid): Simultaneous determination of 8 polybrominated biphenyls in human serum using gas chromatography-mass spectrometry

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DOI	10.3724/SP.J.1123.2011.11055
	Simultaneous determination of 8 polybrominated biphenyls in human serum using gas chromatography-mass spectrometry
	Liu Xiao; Li Jingguang; Huang Feifei; Wu Yongning
通讯作者	Li, JG
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2012
卷号	30 期号:5 页码:468-473
英文摘要	A comprehensive analytical method based on gas chromatography-mass spectrometry (GC-MS) has been developed for the simultaneous determination of 8 polybrominated biphenyl congeners (PBBs: BB-15, 18, 52, 101, 153, 180, 194 and 206) in human serum. After the protein was removed, the sample was cleaned-up by an Oasis (R) HLB solid-phase extraction (SPE) cartridge, then purified further by a two-layer cartridge containing activated silica gel and a mixture of silica gel and sulfuric acid, in which elution solvent was optimized. The eluent was evaporated to about 100 microL by a gentle nitrogen stream for GC-MS analysis. The separation was performed on a DB-5ms column (15 m x 0.25 mm x 0.1 mu m) and the qualitative and quantitative analyses were carried out in electron impact (EI) selected ion monitoring (SIM) mode, in which isotope was used as internal standard. The limits of detection (LODs, 3.14 times of standard deviation) and the limits of quantification (LOQs, 10 times of standard deviation) were 0.002-0.029 ng/mL and 0.008-0.092 ng/mL respectively for the 8 PBBs. The average recoveries for all PBBs at three spiked levels were 74.24%-119.49% with the relative standard deviations in the range of 1.23%-12.02%. The method was verified by accurate analysis of BB-153 in organic contaminant standard reference materials (SRM) 1957 and 1958. This method is simple, rapid, accurate, precise and fit for the determination of PBBs in human serum.
英文关键词	solid-phase extraction (SPE) gas chromatography-mass spectrometry (GC-MS) polybrominated bipbenyls (PBBs) human serum
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435251800007
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330177
作者单位	[Liu Xiao; Li Jingguang] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China; [Liu Xiao; Li Jingguang; Huang Feifei; Wu Yongning] Chinese Ctr Dis Control & Prevent, Natl Inst Nutr & Food Safety, Beijing 100050, Peoples R China; [Huang Feifei] Suzhou Ctr Dis Control & Prevent, Suzhou 215004, Peoples R China
推荐引用方式 GB/T 7714	Liu Xiao,Li Jingguang,Huang Feifei,et al. Simultaneous determination of 8 polybrominated biphenyls in human serum using gas chromatography-mass spectrometry[J],2012,30(5):468-473.
APA	Liu Xiao,Li Jingguang,Huang Feifei,&Wu Yongning.(2012).Simultaneous determination of 8 polybrominated biphenyls in human serum using gas chromatography-mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,30(5),468-473.
MLA	Liu Xiao,et al."Simultaneous determination of 8 polybrominated biphenyls in human serum using gas chromatography-mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 30.5(2012):468-473.