干旱区生态环境知识资源中心(Arid): Determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines using ultra high performance liquid chromatography-triple quadrupole mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2011.01076
	Determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines using ultra high performance liquid chromatography-triple quadrupole mass spectrometry
	Liu Ying; Han Shen; Feng Qian; Wang Jinhua
通讯作者	Wang, JH
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2011
卷号	29 期号:11 页码:1076-1081
英文摘要	A method for the simultaneous determination of aristolochic acids A and B in some Chinese herbals and traditional Chinese patent medicines by ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed. Fourteen samples, including Radix Bupleuri, Radix Glycyrrhizae, Radix Platycodonis, Longdanxieganwan, Xiaopangwan, Slimming Tea, etc., were extracted with methanol-water (70: 30, v/v) and purified with Oasis MAX solid-phase extraction cartridges, then analyzed on an Eclipse RP HD C-18 column (150 mm x 2.1 mm, 1.8 mu m) using 5 mmol/L ammonium acetate solution (pH 7.5)-acetonitrile (75: 25, v/v) as the mobile phase. The mass spectrometric acquisition was carried out by means of electrospray ionization in positive mode (ESI+) with multiple reaction monitoring (MRM) method. The good linearities (r(2) > 0.995) were achieved within the ranges of 0.5 - 200 mu g/L and 1 - 200 mu g/L for aristolochic acids A and B, respectively. The limits of detection (LODs) were 5 mu g/kg for aristolochic A and 7.5 mu g/kg for aristolochic B, and the limits of quantification (LOQs) were 12.5 mu g/kg and 25 mu g/kg, respectively. The recoveries of aristolochic acids A and B at the spiked levels of 100 mu g/kg and 500 mu g/kg ranged from 60.3% to 96.4% with the relative standard deviations (RSDs) not more than 10.2%. The results demonstrated that the proposed method is efficient, sensitive, reproducible, reliable and suitable for the trace determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines.
英文关键词	ultra high performance liquid chromatography-tandem mass spec tromety (UH-PLC-MS/MS) solid-phase extraction (SPE) aristolochic acid tradifional Chinese medicine
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435249900006
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329864
作者单位	[Liu Ying; Han Shen; Feng Qian; Wang Jinhua] Beijing Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Tech Ctr, Beijing 100026, Peoples R China
推荐引用方式 GB/T 7714	Liu Ying,Han Shen,Feng Qian,et al. Determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines using ultra high performance liquid chromatography-triple quadrupole mass spectrometry[J],2011,29(11):1076-1081.
APA	Liu Ying,Han Shen,Feng Qian,&Wang Jinhua.(2011).Determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines using ultra high performance liquid chromatography-triple quadrupole mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,29(11),1076-1081.
MLA	Liu Ying,et al."Determination of aristolochic acids A and B in Chinese herbals and traditional Chinese patent medicines using ultra high performance liquid chromatography-triple quadrupole mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 29.11(2011):1076-1081.