干旱区生态环境知识资源中心(Arid): Determination of polybrominated diphenvl ethers in human serum using solid-phase extraction and gas chromatography coupled with negative chemical ionization mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2011.00743
	Determination of polybrominated diphenvl ethers in human serum using solid-phase extraction and gas chromatography coupled with negative chemical ionization mass spectrometry
	Huang Feifei; Zhao Yunfeng; Li Jiangguang; Wu Yongning
通讯作者	Li, JG
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2011
卷号	29 期号:8 页码:743-749
英文摘要	A simplified analytical method comprised of solid-phase extraction (SPE) and gas chromatography coupled with negative chemical ionization mass spectrometry (GC-NCI/MS) has been developed for the determination of 10 polybrominated diphenyl ethers (PBDEs) congeners in human serum. After the extraction by Oasis (R) HLB custom-made SPE cartridges, the lipids in serum were decomposed by concentrated sulfuric acid directly added on the SPE column. The solvent for protein cleanup and the SPE conditions, such as elution solvent and its volume were optimized. The recoveries of the PBDEs spiked in fetal bovine serum relative to internal standards were in the range of 78.5% - 109.7% at five spiked levels (three spiked levels for BDE-209). The intra-day relative standard deviations (RSDs) were between 0.3% and 7.4%, while the inter-day RSDs were between 1.42% and 14.1%. The limits of detection (LOD, S/N = 3) and the limits of quantification (LOQ, S/N = 10) were in the range of 0.10 - 0.27 ng/L and 0.35 -0.91 ng/L respectively for all PBDEs, except BDE-209. The LOQ (blank concentration value x 3) for BDE-209 was 7.91 ng/L. The method was verified by accurate analysis of organic contaminant standard reference materials (SRM) 1957 and 1958. The results indicated that the proposed method is sensitive, accurate, fast, simple, low solvent consumption and suitable for the determination of tri- to deca-BDE in human serum.
英文关键词	solid-phase extraction (SPE) gas chromatography coupled with negative chemical ionization mass spectrometry (GC-NCl/MS) polybrominated diphenyl ethers (PBDEs) serum
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435249100010
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329863
作者单位	[Huang Feifei; Zhao Yunfeng; Li Jiangguang; Wu Yongning] Chinese Ctr Dis Control & Prevent, Natl Inst Nutr & Food Safety, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Huang Feifei,Zhao Yunfeng,Li Jiangguang,et al. Determination of polybrominated diphenvl ethers in human serum using solid-phase extraction and gas chromatography coupled with negative chemical ionization mass spectrometry[J],2011,29(8):743-749.
APA	Huang Feifei,Zhao Yunfeng,Li Jiangguang,&Wu Yongning.(2011).Determination of polybrominated diphenvl ethers in human serum using solid-phase extraction and gas chromatography coupled with negative chemical ionization mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,29(8),743-749.
MLA	Huang Feifei,et al."Determination of polybrominated diphenvl ethers in human serum using solid-phase extraction and gas chromatography coupled with negative chemical ionization mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 29.8(2011):743-749.