干旱区生态环境知识资源中心(Arid): Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Arid

DOI	10.3724/SP.J.1123.2010.01038
	Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry
	Yan Lijuan; Zhang Feng; Fang Enhua; Guo Yanni; Zhou Yu; Lin Liyi; Chu Xiaogang
通讯作者	Yan, LJ
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2010
卷号	28 期号:11 页码:1038-1042
英文摘要	A method for the simultaneous determination of six macrolide antibiotics (oleandomycin erythromycin, kitasamycin, josamycin, roxithromycin and tylosin) and two lincosamide antibiotics (lincomycin and clindamycin) in animal feeds by ultra-performance liquid chromatography-electrospary ionization tandem mass spectrometry ( UPLC-ESI-MS/MS) was developed. The macrolide and lincosamide antibiotics were extracted from the feeds with methanol followed by enrichment and clean-up with an Oasis HLB cartridge. The UPLC separation was performed on a Waters Acquity UPLC BEH C-18 column by a gradient elution using 0. 1% formic acid and acetonitrile as the mobile phase at a flow rate of 0. 3 mL/min. The identification of eight drugs was carried out by positive electrospray ionization in multiple reaction monitoring (MRM) mode, and the quantification analysis was performed by external standard method. The calibration curves showed good linearity in the range of 1 - 100 mu g/L. The average recoveries of the eight drugs from the feeds spiked at 1, 10 and 100 mu g/kg levels were between 68. 6% and 95. 2%; and the relative standard deviations (RSD) were between 4. 9% and 11. 8%. The limits of quantification (LOQ) of the drugs in the feeds were 1 mu g/kg. The method is simple, rapid, sensitive and suitable for the simultaneous determination of macrolide and lincosamide antibiotics in animal feeds.
英文关键词	ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) macrolide antibiotics lincosamide antibiotics feeds
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435243300006
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329600
作者单位	[Yan Lijuan; Fang Enhua; Guo Yanni; Zhou Yu; Lin Liyi] Xiamen Entry Exit Inspect & Quarantine Bur, Xiamen 361012, Peoples R China; [Zhang Feng; Chu Xiaogang] Chinese Acad Inspect & Quarantine, Beijing 100025, Peoples R China
推荐引用方式 GB/T 7714	Yan Lijuan,Zhang Feng,Fang Enhua,et al. Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry[J],2010,28(11):1038-1042.
APA	Yan Lijuan.,Zhang Feng.,Fang Enhua.,Guo Yanni.,Zhou Yu.,...&Chu Xiaogang.(2010).Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,28(11),1038-1042.
MLA	Yan Lijuan,et al."Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 28.11(2010):1038-1042.