干旱区生态环境知识资源中心(Arid): Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography

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	Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography
	Chen Ai; He Qiaosang; Wang Pingya; Zhou Yong; Huang Li; Xu Zhenjian; Zhao Hua; Zhang Weiying
通讯作者	Wang, PY
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2009
卷号	27 期号:6 页码:804-808
英文摘要	A method was developed for the determination of 6 p-hydroxybenzoates (methyl p-hydroxybenzoate (MHB), ethyl p-hydroxybenzoate (EHB), isopropyl p-hydroxybenzoate (IPHB), propyl p-hydroxybenzoate (PUB), isobutyl p-hydroxybenzoate (IBHB) and butyl p-hydroxybenzoate (BHB)in fruits and jams using the combination of solid-phase extraction and high performance liquid chromatography (SPE-HPLC). Two different extraction solutions and three different mobile phases were tested for p-hydroxybenzoates analysis, and finally ethanol was used as the extraction solvent and methanol-citric acid buffer was selected as the mobile phase. The sample was extracted, and purified by an Oasis HLB solid-phase extraction cartridge, then separated on a Symmetry-C-l8 column and detected at the wave length of 258 nm. The results showed that all the calibration graphs were linear in the concentration range of 0. 1 -20. 0 mg/L (r =0. 999 9). The detection limits and quantification limits were 0. 1 mg/kg (S/N = 3) and 0. 3 mg/kg (S/N = 10) respectively for MHB, EHB, IPHB and PHB, 0. 2 mg/kg (S/N = 3) and 0. 6 mg/kg (s/N = 10) respectively for IBHB and BHB. The average recoveries were between 82. 8% and 115. 5% with the relative standard deviations (RSDs) of 0. 2% -6. 8% (n = 6). The method is simple, rapid, sensitive and reproducible, and can be used for the routine analysis of the p-hydroxybenzoates in fruits and jams.
英文关键词	solid phase extraction (SPE) high performance liquid chromatography (HPLC) p-hydroxybenzoates fruits jams
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000435241600013
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329344
作者单位	[Chen Ai; Wang Pingya; Zhou Yong; Huang Li; Xu Zhenjian; Zhao Hua; Zhang Weiying] Natl Marine Food Qual Supervis & Testing Ctr, Zhoushan 316021, Peoples R China; [He Qiaosang] Zhejiang Prov Food Qual & Safety Test Ctr, Hangzhou 310013, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen Ai,He Qiaosang,Wang Pingya,et al. Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography[J],2009,27(6):804-808.
APA	Chen Ai.,He Qiaosang.,Wang Pingya.,Zhou Yong.,Huang Li.,...&Zhang Weiying.(2009).Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography.CHINESE JOURNAL OF CHROMATOGRAPHY,27(6),804-808.
MLA	Chen Ai,et al."Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography".CHINESE JOURNAL OF CHROMATOGRAPHY 27.6(2009):804-808.