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	Determination of 9 cephalosporin drug residues in beef by ultra performance liquid chromatography-tandem mass spectrometry
	Bai Guotao; Chu Xiaogang; Pan Guoqing; Li Xiuqin; Yong Wei
通讯作者	Chu, XG
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2009
卷号	27 期号:4 页码:417-420
英文摘要	A confirmative method to determine 9 cephalosporin residues in beef by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed. The sample was homogenized and extracted with acetonitrile and water for 1 min at 14 000 r/min, centrifuged at 10 000 r/min and 4 degrees C for 10 min. A total of 2 mL saturated sodium chloride solution was added to avoid foaming during the acetonitrile evaporation , the acetonitrile was evaporated below 37 degrees C using a rotary evaporator, and then cleaned up on an Oasis HLB (500 mg, 6 mL) SPE column by washing with 5 mL water and eluting with acetonitrile-water (7:3, v/v). The eluate was blown to dryness under a stream of nitrogen and adjusted to 3. 0 mL with water. The separation was carried out on an ACQUITY UPLC (TM) BEH C-18. column within 5 min, analyzed by UPLC-MS/MS system with external standard method. The limits of quantification ( LOQs) of cefuroxime ceftiofur and cefalonium were 10, 0. 5 and 0. 5 mu g/kg , respectively; the LOQs of other cephalosporins were 1. 0 mu g/kg. The recoveries of cephalosporins ranged from 74. 2% to 119% and the relative standard deviations (RSDs) ranged from 2. 9% to 15% for the spiked beef sample. The method is quick, easy, very sensitive and suitable for the determination of cephalosporin residues in beef.
英文关键词	ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) cephalosporin beef
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435241200007
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329343
作者单位	[Bai Guotao; Pan Guoqing] Inner Mongolia Entry Exit Inspect & Quarantine Bu, Hohhot 010020, Peoples R China; [Bai Guotao; Chu Xiaogang; Li Xiuqin; Yong Wei] Chinese Acad Inspect & Quarantine, Beijing 100025, Peoples R China
推荐引用方式 GB/T 7714	Bai Guotao,Chu Xiaogang,Pan Guoqing,et al. Determination of 9 cephalosporin drug residues in beef by ultra performance liquid chromatography-tandem mass spectrometry[J],2009,27(4):417-420.
APA	Bai Guotao,Chu Xiaogang,Pan Guoqing,Li Xiuqin,&Yong Wei.(2009).Determination of 9 cephalosporin drug residues in beef by ultra performance liquid chromatography-tandem mass spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,27(4),417-420.
MLA	Bai Guotao,et al."Determination of 9 cephalosporin drug residues in beef by ultra performance liquid chromatography-tandem mass spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 27.4(2009):417-420.