干旱区生态环境知识资源中心(Arid): Determination of ciprofloxacin residue in fish/shellfish tissues using liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique

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	Determination of ciprofloxacin residue in fish/shellfish tissues using liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique
	Chen Xiaohong; Wang Yufei; Yao Xunping; Jin Micong
通讯作者	Jin, MC
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2009
卷号	27 期号:1 页码:39-43
英文摘要	Using isotope internal standard dilution technique, a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed for the identification and quantitative determination of ciprofloxacin residue in the tissues of various fishes/shellfishes. The homogenized tissue sample added with ciprofloxacin-D8 and phosphate buffer solution (pH 7.0) was extracted with acetonitrile under ultrasonication, and degreased with hexane. After solid-phase extraction (SPE) was performed on an Oasis (R) MAX cartridge, the sample was separated on a Cloversil-C18 column (150 mm x 4.6 mm, 5 mu m) by using the mobile phase consisting of CH3CN-0.05% CF3 COOH (25:75, v/v). The detection was carried out by LC-MS/MS using an electrospray ionization interface in multiple reaction monitoring (MRM) mode. The quantification using isotope-labelled internal standard was based on the peak area ratio of ciprofloxacin and deuterated internal standard in the MRM mode. The calibration curve was linear within the range of 0.1 - 50.0 mu g/kg and the limit of quantification was 0.1 mu g/kg (S/N >= or = 10). The recovery was between 92.5% and 98.1%, and the relative standard deviation was less than 4.3%. The application of this method was further demonstrated by analyzing ten various real samples from local markets. The results show that this method is sensitive, accurate and suitable for the confirmative determination of ciprofloxacin residues.
英文关键词	solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) isotope internal standard ciprofloxacin fish/shellfish tissues
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435240700007
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329340
作者单位	[Chen Xiaohong; Wang Yufei; Yao Xunping; Jin Micong] Ningbo Municipal Ctr Dis Control & Prevent, Ningbo Key Lab Poison Res & Control, Ningbo 315010, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen Xiaohong,Wang Yufei,Yao Xunping,et al. Determination of ciprofloxacin residue in fish/shellfish tissues using liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique[J],2009,27(1):39-43.
APA	Chen Xiaohong,Wang Yufei,Yao Xunping,&Jin Micong.(2009).Determination of ciprofloxacin residue in fish/shellfish tissues using liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique.CHINESE JOURNAL OF CHROMATOGRAPHY,27(1),39-43.
MLA	Chen Xiaohong,et al."Determination of ciprofloxacin residue in fish/shellfish tissues using liquid chromatography-tandem mass spectrometry with isotope internal standard dilution technique".CHINESE JOURNAL OF CHROMATOGRAPHY 27.1(2009):39-43.