干旱区生态环境知识资源中心(Arid): The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-beta, NF-kB, PDGF-alpha and Fibronectin in MRC-5 Fibroblast Cells

Arid

DOI	10.4046/trd.2009.67.6.528
	The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-beta, NF-kB, PDGF-alpha and Fibronectin in MRC-5 Fibroblast Cells
	Kim, Ah Hyun; Chon, Suyeon; Yoon, Jin Young; Kim, Yu Jin; Kyung, Sun Young; Lee, Sang Pyo; Park, Jeong Woong; Jeong, Sung Hwan
通讯作者	Jeong, SH
来源期刊	TUBERCULOSIS AND RESPIRATORY DISEASES
ISSN	1738-3536
EISSN	2005-6184
出版年	2009
卷号	67 期号:6 页码:528-535
英文摘要	Background: Dust clouds blown by the wind from the arid deserts of Mongolia and Northeast China are known as Asian dust storms. Ambient particulate matter with a diameter <10 mu m (PM10) is associated with the exacerbation of respiratory diseases and increased mortality of heart and lung disease patients. The fibrotic effects of PM10 of Asian dust to pulmonary fibroblast cells are unknown. This study examined the production of reactive oxygen species (ROS), TGF-beta, NF-kB, PDGF-alpha and Fibronectin in fibroblasts exposed to Asian dust particles. Methods: Air samples were collected using a high volume air sampler (Sibata model HV500F) with an air flow of 500 L/min for at least 6 hours. The MRC-5 cells were exposed to 0, 50 and 100 mu g/mL of PM10 for 24 hours. ROS was detected by measuring the level of oxidized DCF using FACS. TGF-beta, NF-kB, PDGF-alpha and fibronectin were detected by western blotting. Results: There was no increase in the ROS, TGF-beta and PDGF-alpha levels in the MRC-5 cells exposed to PM10. The NF-kB level was higher in the MRC-5 cells exposed to 50 and 100 mu g/mL of PM10 for 24 hours. The fibronectin level in the MRC-5 cells after 24 hours incubation with 50 mu g/mL PM10 was significantly higher than the control group (PM10 50 mu g/mL 113.27 +/- 8.65 of control, p=0.005). Conclusion: PM10 from Asian dust increases the activation of NF-kB and fibronectin expression in MRC-5 fibroblast cells.
英文关键词	Particulate Matter Dust Reactive Oxygen Species Pulmonary Fibrosis Fibroblasts
类型	Article
语种	其他
收录类别	ESCI
WOS记录号	WOS:000420420500006
WOS类目	Respiratory System
WOS研究方向	Respiratory System
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/329295
作者单位	[Kim, Ah Hyun] Gachon Univ Med & Sci, Incheon, South Korea; [Chon, Suyeon; Yoon, Jin Young; Kim, Yu Jin; Kyung, Sun Young; Lee, Sang Pyo; Park, Jeong Woong; Jeong, Sung Hwan] Gachon Univ, Gil Hosp, Dept Internal Med, Div Pumonol, 1198,Kuwol dong, Incheon 405760, South Korea
推荐引用方式 GB/T 7714	Kim, Ah Hyun,Chon, Suyeon,Yoon, Jin Young,et al. The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-beta, NF-kB, PDGF-alpha and Fibronectin in MRC-5 Fibroblast Cells[J],2009,67(6):528-535.
APA	Kim, Ah Hyun.,Chon, Suyeon.,Yoon, Jin Young.,Kim, Yu Jin.,Kyung, Sun Young.,...&Jeong, Sung Hwan.(2009).The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-beta, NF-kB, PDGF-alpha and Fibronectin in MRC-5 Fibroblast Cells.TUBERCULOSIS AND RESPIRATORY DISEASES,67(6),528-535.
MLA	Kim, Ah Hyun,et al."The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-beta, NF-kB, PDGF-alpha and Fibronectin in MRC-5 Fibroblast Cells".TUBERCULOSIS AND RESPIRATORY DISEASES 67.6(2009):528-535.