干旱区生态环境知识资源中心(Arid): Simultaneous Determination of Twelve Glucocorticoids Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry

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DOI	10.1016/S1872-2059(06)60010-2
	Simultaneous Determination of Twelve Glucocorticoids Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry
	Cui Xiaoliang; Shao Bing; Zhao Rong; Meng Juan; Tu Xiaoming
通讯作者	Cui, XL
来源期刊	CHINESE JOURNAL OF CHROMATOGRAPHY
ISSN	1000-8713
出版年	2006
卷号	24 期号:3 页码:213-217
英文摘要	A comprehensive analytical method based on ultra performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS) with negative mode has been developed for the simultaneous determination of twelve glucocorticoids residues in milk. The multi-reaction monitoring mode was employed for the determination. Milk samples were extracted by sonication in a methanol/acetate buffer (pH 5. 20) solution, and then defatted with n-hexane. Sample concentration and purification were performed using Oasis HLB Sep-pak silica and Sep-pak amino-propyl solid phase extraction cartridges. The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column (100 mm x 1. 0 mm i. d., 1. 7 mu m) with gradient elution using methanol and water (containing 0. 1% formic acid) at a flow rate of 0. 1 mL/min. Identification of the glucocorticoids was done using retention times and the distribution of diagnostic ion pairs. Quantification of the glucocorticoids was based on the peak areas of the parent ion and a fragment ion with a higher signal. The limits of detection (LOD) of the method were from 0. 02 to 0. 38 g/kg and the limits of quantification (LOQ) ranged from 0. 07 to 1.27 mu g/kg. Average recoveries for the twelve glucocorticoids (spiked at the levels of 2 and 0. 4 mu g/kg) ranged from 69. 3% to 94. 3%, with relative standard deviations between 3. 5% and 16. 7%. Routine tests showed that the method is fast, sensitive and specific for the determination of glucocorticoids residues in milk.
英文关键词	ultra performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS) solid-phase extraction glucocorticoids residues milk
类型	Article
语种	中文
收录类别	ESCI
WOS记录号	WOS:000435236200001
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328816
作者单位	[Cui Xiaoliang] Tianjin Univ, Dept Chem, Tianjin 300072, Peoples R China; [Cui Xiaoliang; Shao Bing; Zhao Rong; Meng Juan; Tu Xiaoming] Beijing Ctr Dis Prevent & Control, Inst Nutr & Food Hyg, Beijing 100013, Peoples R China
推荐引用方式 GB/T 7714	Cui Xiaoliang,Shao Bing,Zhao Rong,et al. Simultaneous Determination of Twelve Glucocorticoids Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry[J],2006,24(3):213-217.
APA	Cui Xiaoliang,Shao Bing,Zhao Rong,Meng Juan,&Tu Xiaoming.(2006).Simultaneous Determination of Twelve Glucocorticoids Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry.CHINESE JOURNAL OF CHROMATOGRAPHY,24(3),213-217.
MLA	Cui Xiaoliang,et al."Simultaneous Determination of Twelve Glucocorticoids Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry".CHINESE JOURNAL OF CHROMATOGRAPHY 24.3(2006):213-217.