干旱区生态环境知识资源中心(Arid): Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway

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DOI	10.1111/phen.12346
	Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway
	Miladi, Meriam; Abdellaoui, Khemais; Marchal, Elisabeth; Lenaerts, Cynthia; Ben Hamouda, Amel; M'saad Guerfali, Meriem; Ben Halima Kamel, Monia; Vanden Broeck, Jozef
通讯作者	Abdellaoui, K
来源期刊	PHYSIOLOGICAL ENTOMOLOGY
ISSN	0307-6962
EISSN	1365-3032
英文摘要	Fractionation of the methanolic extract of Pergularia tomentosa, using column chromatography, resulted in the separation of 10 fractions and only the active one (that gave mortality >70%) was kept and denoted the active fraction (AFPt). Adult female Schistocerca gregaria were treated with two concentrations of AFPt, 0.24% and 0.96%, to investigate the effect on the female reproductive potential. Both concentrations of AFPt significantly increased the duration between adult emergence and first oviposition, as well as affected fecundity and fertility. Furthermore, the effects of AFPt on vitellogenesis and ecdysteroid titres were examined. Biochemical analyses revealed a significant decrease in protein, lipid and carbohydrate contents in ovaries. This study also showed that AFPt induced a significant decrease in the ovarian ecdysteroids levels, as well as the haemolymph ecdysteroid titres. The transcript levels of the ecdysteroid synthesis enzymes Spook and Shadow were significantly decreased when treated with the highest concentration of AFPt, while transcript levels of the ecdysteroid synthesis enzymes Phantom and Disembodied increased significantly in animals treated with AFPt. Treatment with AFPt also resulted in a significantly higher transcript level of both the ecdysone receptor (EcR) and its partner ultraspiracle/retinoid-X-receptor (USP/RXR).
英文关键词	Ecdysone receptor Halloween genes locust Pergularia tomentosa reproduction retinoid‐ X‐ receptor
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000598290100001
WOS关键词	ENZYME-IMMUNOASSAY ; ECDYSONE RECEPTOR ; ESSENTIAL OILS ; BIOSYNTHESIS ; REPRODUCTION ; CARDENOLIDES ; EXTRACT ; GENES ; ECR
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328597
作者单位	[Miladi, Meriam; Abdellaoui, Khemais; Ben Hamouda, Amel; Ben Halima Kamel, Monia] Sousse Univ, Higher Agron Inst Chott Mariem, Sousse, Tunisia; [Marchal, Elisabeth; Lenaerts, Cynthia; Vanden Broeck, Jozef] Katholieke Univ Leuven, Mol Dev Physiol & Signal Transduct, Leuven, Belgium; [M'saad Guerfali, Meriem] Technopole Sidi Thabet, Natl Ctr Nucl Sci & Technol, Lab Biotechnol & Nucl Technol, LR16CNSTN01, Tunis, Tunisia
推荐引用方式 GB/T 7714	Miladi, Meriam,Abdellaoui, Khemais,Marchal, Elisabeth,et al. Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway[J].
APA	Miladi, Meriam.,Abdellaoui, Khemais.,Marchal, Elisabeth.,Lenaerts, Cynthia.,Ben Hamouda, Amel.,...&Vanden Broeck, Jozef.
MLA	Miladi, Meriam,et al."Effects of Pergularia tomentosa active fraction on the oogenesis of the desert locust, Schistocerca gregaria: ovarian biochemical composition, and effects on the ecdysteroid pathway".PHYSIOLOGICAL ENTOMOLOGY