干旱区生态环境知识资源中心(Arid): Simultaneous determination of paraquat and diquat in human plasma by HPLC-DAD: Its application in acute poisoning patients induced by these two herbicides

Arid

DOI	10.1002/jcla.23669
	Simultaneous determination of paraquat and diquat in human plasma by HPLC-DAD: Its application in acute poisoning patients induced by these two herbicides
	Yuan, Guiyan; Li, Rong; Zhao, Qi; Kong, Xianglin; Wang, Yanyan; Wang, Xiaojing; Guo, Ruichen
通讯作者	Guo, RC
来源期刊	JOURNAL OF CLINICAL LABORATORY ANALYSIS
ISSN	0887-8013
EISSN	1098-2825
英文摘要	Background Paraquat and diquat are widely used in agricultural production in many countries, which are very toxic to human beings. Paraquat can be detected in some diquat solution sold in the market. The blood concentration of paraquat or diquat is an important indicator for clinical diagnosis of paraquat or diquat poisoning. So, it is very meaningful to develop a method for simultaneous determination of paraquat and diquat in human plasma. Objective To develop and validate a HPLC-DAD method for simultaneous determination of paraquat and diquat in human plasma and to apply it in the acute poisoning patients by these two herbicides. Methods Paraquat and diquat were simultaneously determined by HPLC-DAD. The plasma was treated using Waters OASIS(R) Column and then separated on a Thermo Hypersil GOLD (250 x 4.6 mm, 5 mu m) Column with the mobile phase consisted of 75 mmol/L sodium heptane sulfonate (containing 0.1 mol/L phosphoric acid, pH 3.0) and acetonitrile (87:13, v:v) at a flow rate of 1.0 mL/min. The full-wavelength scanning was 200-400 nm, and the detection wavelength of paraquat and diquat was 257nm and 310nm, respectively. 120 and 30 plasma samples from patients with paraquat and diquat poisoning were collected and analyzed by the established method. Results The standard curve for paraquat and diquat ranged from 0.05 to 20 mu g/mL, and the precision of LLOQ for paraquat was 16.49%, which was required to be less than 20%. The precision of other concentrations was less than 14.14%. The recovery of paraquat and diquat was 95.38%-103.97% and 94.79%-98.40%, respectively. The results showed that paraquat and diquat were stable under various storage conditions. 120 plasma samples of paraquat poisoning patients and 30 plasma samples of diquat poisoning patients were determined by the established method. The blood concentration of paraquat ranged from 0.10 to 20.62 mu g/mL, with an average of 3.61 mu g/mL, while for diquat, the concentration ranged from 0 to 26.59 mu g/mL, with an average of 2.00 mu g/mL. Among the diquat suspected poisoning samples, 5 samples were detected not only diquat but also paraquat, and 2 samples were detected only paraquat, no diquat. Conclusion The HPLC-DAD method established in this study was high throughput, high sensitivity, simple operation, and wide linear ranges. It can be used for the screening analysis and quantitative detection of paraquat and diquat in acute poisoning patients, which can provide basis for the treatment and prognosis of these two herbicides poisoning patients.
英文关键词	acute poisoning patients Diquat high‐ performance liquid chromatography Paraquat simultaneous determination
类型	Article ; Early Access
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000596681300001
WOS类目	Medical Laboratory Technology
WOS研究方向	Medical Laboratory Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328585
作者单位	[Yuan, Guiyan; Li, Rong; Zhao, Qi; Kong, Xianglin; Wang, Yanyan; Guo, Ruichen] Shandong Univ, Dept Pharm, Qilu Hosp, 107 Wenhua West Rd, Jinan 250012, Peoples R China; [Wang, Xiaojing] Shandong Med Coll, Dept Lab Med, 5460 Erhuan South Rd, Jinan 250002, Shandong, Peoples R China; [Zhao, Qi] Shandong Rongjun Hosp, Dept Pharm, Jinan, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Guiyan,Li, Rong,Zhao, Qi,et al. Simultaneous determination of paraquat and diquat in human plasma by HPLC-DAD: Its application in acute poisoning patients induced by these two herbicides[J].
APA	Yuan, Guiyan.,Li, Rong.,Zhao, Qi.,Kong, Xianglin.,Wang, Yanyan.,...&Guo, Ruichen.
MLA	Yuan, Guiyan,et al."Simultaneous determination of paraquat and diquat in human plasma by HPLC-DAD: Its application in acute poisoning patients induced by these two herbicides".JOURNAL OF CLINICAL LABORATORY ANALYSIS