Arid
DOI10.1080/09583157.2020.1843599
Production of conidia by entomopathogenic isolates of Fusarium caatingaense on different vegetable substrates
de Lima, Isabela Janne; Carneiro Leao, Mariele Porto; da Silva Santos, Ana Carla; da Costa, Antonio Felix; Tiago, Patricia Vieira
通讯作者Tiago, PV
来源期刊BIOCONTROL SCIENCE AND TECHNOLOGY
ISSN0958-3157
EISSN1360-0478
英文摘要The cactus Opuntia ficus-indica (L.) Miller, used as the principal food source for livestock in arid and semi-arid regions of Brazil, is being threatened by cochineal scale, Dactylopius opuntiae Cockerell. The potential of entomopathogenic isolates of Fusarium caatingaense Santos, Lima, Tiago & Oliveira to control this pest has been reported. The objective of this study was to select suitable substrates for the production of conidia by F. caatingaense isolates, while maintaining their viability and pathogenicity against the insect. Five F. caatingaense isolates obtained from D. opuntiae were grown on 11 substrates: rice grains, rice grains + peptone, cracked corn, cracked corn + peptone, cracked corn + yeast extract, cactus flour, cactus flour + peptone, wheat bran, wheat bran + peptone, castor bean meal, and castor bean meal + peptone. The production and viability of conidia of the F. caatingaense isolates varied among the vegetable substrates studied. Rice grains, cracked corn and wheat bran, with or without supplementation, were the best substrates, especially for the URM 6779 and URM 6782 isolates. The URM 6779 isolate was selected for sporulation, germination and pathogenicity analysis after storage in rice at different temperatures (4 and 28 degrees C) and time periods (0, 15, 30, 60 and 90 days). Storage at 4 degrees C was the most suitable for mass production of this fungus because it maintained good rates of production and germination of conidia, and mortality against D. opuntiae until the 90th day of storage.
英文关键词Entomopathogenic fungi mass production sporulation viability pathogenicity
类型Article ; Early Access
语种英语
收录类别SCI-E
WOS记录号WOS:000591465800001
WOS关键词EQUISETI SPECIES COMPLEX ; DACTYLOPIUS-OPUNTIAE ; MASS-PRODUCTION ; SCALE PRODUCTION ; FUNGI ; SELECTION ; EXTRACTS ; AGENTS
WOS类目Biotechnology & Applied Microbiology ; Entomology
WOS研究方向Biotechnology & Applied Microbiology ; Entomology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/328526
作者单位[de Lima, Isabela Janne; da Silva Santos, Ana Carla; Tiago, Patricia Vieira] Univ Fed Pernambuco, Dept Micol, Rua Engn S-N,Cidade Univ, BR-50740600 Recife, PE, Brazil; [Carneiro Leao, Mariele Porto; da Costa, Antonio Felix] Inst Agron Pernambuco, Recife, PE, Brazil
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de Lima, Isabela Janne,Carneiro Leao, Mariele Porto,da Silva Santos, Ana Carla,et al. Production of conidia by entomopathogenic isolates of Fusarium caatingaense on different vegetable substrates[J].
APA de Lima, Isabela Janne,Carneiro Leao, Mariele Porto,da Silva Santos, Ana Carla,da Costa, Antonio Felix,&Tiago, Patricia Vieira.
MLA de Lima, Isabela Janne,et al."Production of conidia by entomopathogenic isolates of Fusarium caatingaense on different vegetable substrates".BIOCONTROL SCIENCE AND TECHNOLOGY
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