干旱区生态环境知识资源中心(Arid): Predominance of Burkholderia cenocepacia lineages causing onion sour skin in the semi-arid region of north-east Brazil

Arid

DOI	10.1111/ppa.13311
	Predominance of Burkholderia cenocepacia lineages causing onion sour skin in the semi-arid region of north-east Brazil
	Baia, Ana D. B.; Silva, Adriano M. F.; Ribeiro, Barbara G.; Souza, Claudeana C.; Silva Junior, Wilson J.; Balbino, Valdir Q.; Leal, Carla M.; de Farias, Antonio R. G.; Souza, Elineide B.; Gama, Marco A. S.
通讯作者	Gama, MAS
来源期刊	PLANT PATHOLOGY
ISSN	0032-0862
EISSN	1365-3059
英文摘要	Burkholderia cepacia was originally described as the causal agent of onion sour skin. However, this bacterium is now recognized as a complex of 22 closely related species, commonly referred to as the B. cepacia complex (BCC). Only a few taxonomic studies have been undertaken with the aim of understanding the diversity of species associated with onion sour skin. In this study, we used a polyphasic analysis including rep-PCR assay, biochemical and pathological profiles, and multilocus sequence analysis (MLSA) to characterize the BCC species associated with onion sour skin in the semi-arid region of north-east Brazil. Phylogenetic analysis of the recA of strains representing the genetic variability, as determined by rep-PCR, grouped the strains into four clades. Clades I and II represent B. cenocepacia lineages IIB and IIIA, respectively, while the strains in Clades III and IV clustered more closely to Clades I and II than to strains of any other BCC species. MLSA confirmed the existence of the four clades with a 1.00 posterior probability. A distance matrix indicated a low level of divergence among the strains of the four clades found in the MLSA, proving that B. cenocepacia lineages IIIA, IIIB, and a new lineage of B. cenocepacia are associated with onion sour skin in the semi-arid region of north-east Brazil. Also, surprisingly, no strain was identified as B. cepacia, indicating that this species probably does not play a role in this disease in this region.
英文关键词	Burkholderia cepacia complex multilocus sequence analysis onion crop polyphasic characterization
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000590616300001
WOS关键词	CYSTIC-FIBROSIS PATIENTS ; MULTIPLE SEQUENCE ALIGNMENT ; CEPACIA COMPLEX ; SP-NOV ; IDENTIFICATION ; MULTIVORANS ; GENOMOVARS ; CHOICE
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328512
作者单位	[Baia, Ana D. B.; Ribeiro, Barbara G.; Souza, Claudeana C.; de Farias, Antonio R. G.; Gama, Marco A. S.] Univ Fed Rural Pernambuco, Dept Agron, BR-51030230 Recife, PE, Brazil; [Silva, Adriano M. F.] Univ Fed Alagoas, Dept Agron, Rio Largo, AL, Brazil; [Silva Junior, Wilson J.; Balbino, Valdir Q.] Univ Fed Pernambuco, Dept Genet, Recife, PE, Brazil; [Leal, Carla M.] Georg August Univ Gottingen, Crop Sci Dept, Gottingen, LS, Germany; [Souza, Elineide B.] Univ Fed Rural Pernambuco, Dept Biol, Recife, PE, Brazil
推荐引用方式 GB/T 7714	Baia, Ana D. B.,Silva, Adriano M. F.,Ribeiro, Barbara G.,et al. Predominance of Burkholderia cenocepacia lineages causing onion sour skin in the semi-arid region of north-east Brazil[J].
APA	Baia, Ana D. B..,Silva, Adriano M. F..,Ribeiro, Barbara G..,Souza, Claudeana C..,Silva Junior, Wilson J..,...&Gama, Marco A. S..
MLA	Baia, Ana D. B.,et al."Predominance of Burkholderia cenocepacia lineages causing onion sour skin in the semi-arid region of north-east Brazil".PLANT PATHOLOGY